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Journal of Virology, February 2006, p. 2013-2018, Vol. 80, No. 4
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.4.2013-2018.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Dual Wavelength Imaging Allows Analysis of Membrane Fusion of Influenza Virus inside Cells

Tatsuya Sakai,1* Masanobu Ohuchi,1 Masaki Imai,2 Takafumi Mizuno,3 Kazunori Kawasaki,3 Kazumichi Kuroda,4 and Shohei Yamashina5

Department of Microbiology, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan,1 Department of Virology 3, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo 208-0011, Japan,2 Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan,3 Department of Immunology and Microbiology, Nihon University School of Medicine, Itabashi-ku, Tokyo 173-8610, Japan,4 Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 228-8555, Japan5

Received 29 August 2005/ Accepted 2 December 2005

Influenza virus hemagglutinin (HA) is a determinant of virus infectivity. Therefore, it is important to determine whether HA of a new influenza virus, which can potentially cause pandemics, is functional against human cells. The novel imaging technique reported here allows rapid analysis of HA function by visualizing viral fusion inside cells. This imaging was designed to detect fusion changing the spectrum of the fluorescence-labeled virus. Using this imaging, we detected the fusion between a virus and a very small endosome that could not be detected previously, indicating that the imaging allows highly sensitive detection of viral fusion.

* Corresponding author. Mailing address: Department of Microbiology, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan. Phone: 81-86-462-1111. Fax: 81-86-462-1199. E-mail: sakai{at}med.kawasaki-m.ac.jp.

Journal of Virology, February 2006, p. 2013-2018, Vol. 80, No. 4
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.4.2013-2018.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Imai, M., Mizuno, T., Kawasaki, K. (2006). Membrane Fusion by Single Influenza Hemagglutinin Trimers: KINETIC EVIDENCE FROM IMAGE ANALYSIS OF HEMAGGLUTININ-RECONSTITUTED VESICLES. J. Biol. Chem. 281: 12729-12735 [Abstract] [Full Text]  


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