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Journal of Virology, December 2006, p. 12086-12094, Vol. 80, No. 24
0022-538X/06/$08.00+0     doi:10.1128/JVI.01184-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of an Essential Domain in the Herpesvirus VP1/2 Tegument Protein: the Carboxy Terminus Directs Incorporation into Capsid Assemblons{triangledown} ,{dagger}

Joy I-Hsuan Lee,1,{ddagger} G. W. Gant Luxton,2,{ddagger} and Gregory Allan Smith1*

Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611,1 Department of Anatomy and Cell Biology, Columbia University, New York, New York 100322

Received 7 June 2006/ Accepted 19 September 2006

The herpesvirus tegument is a layer of viral and cellular proteins located between the capsid and envelope of the virion. The VP1/2 tegument protein is critical for the propagation of all herpesviruses examined. Using an infectious clone of the alphaherpesvirus pseudorabies virus, we have made a collection of truncation and in-frame deletion mutations within the VP1/2 gene (UL36) and examined the resulting viruses for spread between cells. We found that the majority of the VP1/2 protein either was essential for virus propagation or did not tolerate large deletions. A recently described amino-terminal deubiquitinase-encoding domain was dispensable for alphaherpesvirus propagation, but the rate of propagation in an epithelial cell line and the frequency of transport in axons of primary sensory neurons were both reduced. We mapped one essential domain to a conserved sequence at the VP1/2 carboxy terminus and demonstrated that this domain sufficient to redirect the green fluorescent protein to capsid assemblons in nuclei of infected cells.

* Corresponding author. Mailing address: Department of Microbiology-Immunology, Ward Bldg., Rm. 10-105, Northwestern University Feinberg School of Medicine, Chicago, IL 60611. Phone: (312) 503-3745. Fax: (312) 503-1339. E-mail: g-smith3{at}northwestern.edu.

{triangledown} Published ahead of print on 27 September 2006.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

{ddagger} J.I.L. and G.W.G.L. contributed equally to this study.

Journal of Virology, December 2006, p. 12086-12094, Vol. 80, No. 24
0022-538X/06/$08.00+0     doi:10.1128/JVI.01184-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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