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Journal of Virology, December 2006, p. 12049-12059, Vol. 80, No. 24
0022-538X/06/$08.00+0     doi:10.1128/JVI.01652-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Visualization of -Helices in a 6-Ångstrom Resolution Cryoelectron Microscopy Structure of Adenovirus Allows Refinement of Capsid Protein Assignments ^,

Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 2215 Garland Avenue, Nashville, Tennessee 37232,¹ Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, IMM-19, La Jolla, California 92037²

Received 1 August 2006/ Accepted 15 September 2006

The structure of adenovirus was determined to a resolution of 6 Å by cryoelectron microscopy (cryoEM) single-particle image reconstruction. Docking of the hexon and penton base crystal structures into the cryoEM density established that -helices of 10 or more residues are resolved as rods. A difference map was calculated by subtracting a pseudoatomic capsid from the cryoEM reconstruction. The resulting density was analyzed in terms of observed -helices and secondary structure predictions for the additional capsid proteins that currently lack atomic resolution structures (proteins IIIa, VI, VIII, and IX). Protein IIIa, which is predicted to be highly -helical, is assigned to a cluster of helices observed below the penton base on the inner capsid surface. Protein VI is present in 1.5 copies per hexon trimer and is predicted to have two long -helices, one of which appears to lie inside the hexon cavity. Protein VIII is cleaved by the adenovirus protease into two fragments of 7.6 and 12.1 kDa, and the larger fragment is predicted to have one long -helix, in agreement with the observed density for protein VIII on the inner capsid surface. Protein IX is predicted to have one long -helix, which also has a strongly indicated propensity for coiled-coil formation. A region of density near the facet edge is now resolved as a four-helix bundle and is assigned to four copies of the C-terminal -helix from protein IX.

Published ahead of print on 27 September 2006.

Supplemental material for this article may be found at http://jvi.asm.org/.

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