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Journal of Virology, November 2006, p. 11381-11384, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.01328-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Papillomavirus Particles Assembled in 293TT Cells Are Infectious In Vivo{triangledown}

Timothy D. Culp,1 Nancy M. Cladel,1 Karla K. Balogh,1 Lynn R. Budgeon,1 Andres F. Mejia,2 and Neil D. Christensen1*

Gittlen Cancer Research Foundation,1 Department of Comparative Medicine, College of Medicine, Pennsylvania State University, Hershey, Pennsylvania2

Received 23 June 2006/ Accepted 23 August 2006

Papillomaviruses (PVs) demonstrate both tissue and species tropisms. Because PVs replicate only in terminally differentiating epithelium, the recent production of infectious PV particles in 293 cells marks an important breakthrough. In this article, we demonstrate that infectious PV particles produced in 293TT cells can cause papillomatous growths in the natural host animal. Moreover, we show that species-matched PV genomes can be successfully delivered in vivo by a heterologous, species-mismatched PV capsid. Additionally, our results indicate that the addition of the simian virus 40 origin of replication to the papillomavirus genome increases the production of infectious papillomavirus particles by increasing genome amplification in the transfected 293TT cells.

* Corresponding author. Mailing address: Jake Gittlen Cancer Research Foundation, College of Medicine, Pennsylvania State University, Hershey, PA 17033-2390. Phone: (717) 531-6185. Fax: (717) 531-5634. E-mail: ndc1{at}psu.edu.

{triangledown} Published ahead of print on 30 August 2006.

Journal of Virology, November 2006, p. 11381-11384, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.01328-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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