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Journal of Virology, November 2006, p. 11074-11081, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.01150-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Differential Biophysical Properties of Infectious Intracellular and Secreted Hepatitis C Virus Particles{triangledown} ,{dagger}

Pablo Gastaminza, Sharookh B. Kapadia, and Francis V. Chisari*

Department of Molecular and Experimental Medicine, the Scripps Research Institute, La Jolla, California 92037

Received 2 June 2006/ Accepted 24 August 2006

The recent development of a cell culture infection model for hepatitis C virus (HCV) permits the production of infectious particles in vitro. In this report, we demonstrate that infectious particles are present both within the infected cells and in the supernatant. Kinetic analysis indicates that intracellular particles constitute precursors of the secreted infectious virus. Ultracentrifugation analyses indicate that intracellular infectious viral particles are similar in size (~65 to 70 nm) but different in buoyant density (~1.15 to 1.20 g/ml) from extracellular particles (~1.03 to 1.16 g/ml). These results indicate that infectious HCV particles are assembled intracellularly and that their biochemical composition is altered during viral egress.

* Corresponding author. Mailing address: The Scripps Research Institute, Maildrop SBR-1010550, North Torrey Pines Road, La Jolla, CA 92037. Phone: (858) 784-8228. Fax: (858) 784-2160. E-mail: fchisari{at}scripps.edu.

{triangledown} Published ahead of print on 6 September 2006.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, November 2006, p. 11074-11081, Vol. 80, No. 22
0022-538X/06/$08.00+0     doi:10.1128/JVI.01150-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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