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Journal of Virology, October 2006, p. 10281-10284, Vol. 80, No. 20
0022-538X/06/$08.00+0     doi:10.1128/JVI.00361-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Alternate Polypurine Tracts Affect Rous Sarcoma Virus Integration In Vivo{dagger}

Jangsuk Oh,1 Kevin W. Chang,1 W. Gregory Alvord,2 and Stephen H. Hughes1*

HIV Drug Resistance Program,1 Data Management Services, National Cancer Institute at Frederick, Frederick, Maryland 21702-12012

Received 21 February 2006/ Accepted 31 July 2006

When the endogenous polypurine tract (PPT) of the Rous sarcoma virus (RSV)-derived vector RSVP(A)Z was replaced with alternate retroviral PPTs, the fraction of unintegrated viral DNA with the normal consensus ends significantly decreased and the retention of part of the PPT significantly increased. If the terminus of the U3 long terminal repeat (LTR) is aberrant, RSV integrase can correctly process and integrate the normal U5 LTR into the host genome. However, the canonical CA is not involved in joining the aberrant U3 LTR to the host DNA, generating either large duplications or deletions of the host sequences instead of the normal 5- or 6-bp duplication.

* Corresponding author. Mailing address: HIV Drug Resistance Program, NCI at Frederick, P.O. Box B, Bldg. 539, Rm. 130A, Frederick, MD 21702-1201. Phone: (301) 846-1619. Fax: (301) 846-6966. E-mail: Hughes{at}ncifcrf.gov.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, October 2006, p. 10281-10284, Vol. 80, No. 20
0022-538X/06/$08.00+0     doi:10.1128/JVI.00361-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Oh, J., Chang, K. W., Hughes, S. H. (2008). Integration of Rous Sarcoma Virus DNA: a CA Dinucleotide Is Not Required for Integration of the U3 End of Viral DNA. J. Virol. 82: 11480-11483 [Abstract] [Full Text]  
  • Oh, J., Chang, K. W., Wierzchoslawski, R., Alvord, W. G., Hughes, S. H. (2008). Rous Sarcoma Virus (RSV) Integration In Vivo: a CA Dinucleotide Is Not Required in U3, and RSV Linear DNA Does Not Autointegrate. J. Virol. 82: 503-512 [Abstract] [Full Text]  


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