This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gonzalez, R.
Right arrow Articles by Flint, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gonzalez, R.
Right arrow Articles by Flint, S. J.

 Previous Article  |  Next Article 

Journal of Virology, January 2006, p. 964-974, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.964-974.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Adenovirus E1B 55-Kilodalton Protein Is Required for both Regulation of mRNA Export and Efficient Entry into the Late Phase of Infection in Normal Human Fibroblasts

Ramon Gonzalez,1,{dagger} Wenying Huang, Renee Finnen,1,{ddagger} Courtney Bragg, and S. J. Flint*

Department of Molecular Biology, Princeton University, Princeton, New Jersey 085441

Received 30 August 2005/ Accepted 27 October 2005

The human adenovirus type 5 (Ad5) E1B 55-kDa protein is required for selective nuclear export of viral late mRNAs from the nucleus and concomitant inhibition of export of cellular mRNAs in HeLa cells and some other human cell lines, but its contributions(s) to replication in normal human cells is not well understood. We have therefore examined the phenotypes exhibited by viruses carrying mutations in the E1B 55-kDa protein coding sequence in normal human fibroblast (HFFs). Ad5 replicated significantly more slowly in HFFs than it does in tumor cells, a difference that is the result of delayed entry into the late phase of infection. The A143 mutation, which specifically impaired export of viral late mRNAs from the nucleus in infected HeLa cells (R. A. Gonzalez and S. J. Flint, J. Virol. 76:4507-4519, 2002), induced a more severe defect in viral mRNA export in HFFs. This observation indicates that the E1B 55-kDa protein regulates mRNA export during the late phase of infection of normal human cells. Other mutants exhibited phenotypes not observed in HeLa cells. In HFFs infected by the null mutant Hr6, synthesis of viral late mRNAs and proteins was severely impaired. Such defects in late gene expression were the result of inefficient progression into the late phase of infection, for viral DNA synthesis was 10-fold less efficient in Hr6-infected HFFs than in cells infected by Ad5. Similar, but less severe, defects in viral DNA synthesis were induced by the insertion mutation H224, which has been reported to inhibit binding of the E1B 55-kDa protein to p53 (C. C. Kao, P. R. Yew, and A. J. Berk, Virology 179:806-814, 1990).

* Corresponding author. Mailing address: Department of Molecular Biology, Princeton University, Princeton, NJ 08544. Phone: (609) 258-6113. Fax: (609) 258-4575. E-mail: sjflint{at}molbio.princeton.edu.

{dagger} Present address: School of Sciences, Autonomous State University of Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Mor. 62210, Mexico.

{ddagger} Present address: Department of Pediatrics, Infectious Diseases Division, Biomedical Research Building 851F, C227, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Denver, CO 80262.

Journal of Virology, January 2006, p. 964-974, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.964-974.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Blackford, A. N., Grand, R. J. A. (2009). Adenovirus E1B 55-Kilodalton Protein: Multiple Roles in Viral Infection and Cell Transformation. J. Virol. 83: 4000-4012 [Full Text]  
  • Miller, D. L., Rickards, B., Mashiba, M., Huang, W., Flint, S. J. (2009). The Adenoviral E1B 55-Kilodalton Protein Controls Expression of Immune Response Genes but Not p53-Dependent Transcription. J. Virol. 83: 3591-3603 [Abstract] [Full Text]  
  • Marshall, L. J., Moore, A. C., Ohki, M., Kitabayashi, I., Patterson, D., Ornelles, D. A. (2008). RUNX1 Permits E4orf6-Directed Nuclear Localization of the Adenovirus E1B-55K Protein and Associates with Centers of Viral DNA and RNA Synthesis. J. Virol. 82: 6395-6408 [Abstract] [Full Text]  
  • Zheng, X., Rao, X.-M., Gomez-Gutierrez, J. G., Hao, H., McMasters, K. M., Zhou, H. S. (2008). Adenovirus E1B55K Region Is Required To Enhance Cyclin E Expression for Efficient Viral DNA Replication. J. Virol. 82: 3415-3427 [Abstract] [Full Text]  
  • Blanchette, P., Kindsmuller, K., Groitl, P., Dallaire, F., Speiseder, T., Branton, P. E., Dobner, T. (2008). Control of mRNA Export by Adenovirus E4orf6 and E1B55K Proteins during Productive Infection Requires E4orf6 Ubiquitin Ligase Activity. J. Virol. 82: 2642-2651 [Abstract] [Full Text]  
  • Kindsmuller, K., Groitl, P., Hartl, B., Blanchette, P., Hauber, J., Dobner, T. (2007). Intranuclear targeting and nuclear export of the adenovirus E1B-55K protein are regulated by SUMO1 conjugation. Proc. Natl. Acad. Sci. USA 104: 6684-6689 [Abstract] [Full Text]  
  • Rickards, B., Flint, S. J., Cole, M. D., LeRoy, G. (2007). Nucleolin Is Required for RNA Polymerase I Transcription In Vivo. Mol. Cell. Biol. 27: 937-948 [Abstract] [Full Text]  
  • Woo, J. L., Berk, A. J. (2007). Adenovirus Ubiquitin-Protein Ligase Stimulates Viral Late mRNA Nuclear Export. J. Virol. 81: 575-587 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»