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Journal of Virology, January 2006, p. 920-928, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.920-928.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Human Cytomegalovirus Immediate-Early 2 Protein IE86 Blocks Virus-Induced Chemokine Expression

R. Travis Taylor{dagger} and Wade A. Bresnahan*

Department of Microbiology, University of Minnesota, 1060 Mayo Building, MMC196, Minneapolis, Minnesota 55455

Received 17 August 2005/ Accepted 18 October 2005

The effect of human cytomegalovirus (HCMV) gene expression on cytokine (beta interferon) and chemokine (RANTES, MIG, MCP-2, MIP-1{alpha}, and interleukin-8) expression was examined. We demonstrate that HCMV gene expression is required to suppress the transcriptional induction of these cytokines and that the HCMV immediate-early 2 gene product IE86 can effectively block the expression of cytokines and proinflammatory chemokines during HCMV and Sendai virus infection. Additionally, we present data on viral mutants and ectopic protein expression which demonstrate that pp65, another identified HCMV cytokine antagonist, is not involved in regulating these proinflammatory cytokines. This is the first report to demonstrate that IE86 can act to suppress virus-induced proinflammatory cytokine transcript expression, extending the antiviral properties of this multifunctional viral protein.

* Corresponding author. Mailing address: Department of Microbiology, University of Minnesota, 1060 Mayo Building, MMC196, Minneapolis, MN 55455. Phone: (612) 626-5876. Fax: (612) 626-0623. E-mail: bresn013{at}umn.edu.

{dagger} Present address: UT Southwestern Medical Center Department of Microbiology, 6000 Harry Hines Blvd., Dallas, TX 75390.

Journal of Virology, January 2006, p. 920-928, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.920-928.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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