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Journal of Virology, January 2006, p. 1032-1037, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.1032-1037.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Interferon Regulatory Factor 3 Is Necessary for Induction of Antiviral Genes during Human Cytomegalovirus Infection{dagger}

Victor R. DeFilippis, Bridget Robinson, Thomas M. Keck, Scott G. Hansen, Jay A. Nelson, and Klaus J. Früh*

Vaccine and Gene Therapy Institute, Oregon Health and Science University, Portland, Oregon

Received 27 July 2005/ Accepted 12 October 2005

Viral infection activates interferon regulatory factor 3 (IRF3), a cofactor for the induction of interferon-stimulated genes (ISGs). The role of IRF3 in the activation of ISGs by human cytomegalovirus (HCMV) is controversial despite the fact that HCMV has consistently been shown to induce ISGs during infection of fibroblasts. To address the function of IRF3 in HCMV-mediated ISG induction, we monitored ISG expression and global gene expression in HCMV-infected cells in which IRF3 function had been depleted by small interfering RNA or blocked by dominant negative IRF3. A specific reduction of ISG induction was observed, whereas other transcripts were unaffected. We therefore conclude that IRF3 specifically regulates ISG induction during the initial phase of HCMV infection.

* Corresponding author. Mailing address: Oregon Health and Science University, West Campus, Vaccine and Gene Therapy Institute, 505 NW 185th Ave., Beaverton, OR 97006. Phone: (503) 418-2735. Fax: (503) 418-2719. E-mail: fruehk{at}ohsu.edu.

{dagger} Supplemental material for this article may be found at http://jvi.asm.org/.

Journal of Virology, January 2006, p. 1032-1037, Vol. 80, No. 2
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.2.1032-1037.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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