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 Previous Article

Journal of Virology, October 2006, p. 9921-9925, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.00380-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Receptor-Triggered but Alkylation-Arrested Env of Murine Leukemia Virus Reveals the Transmembrane Subunit in a Prehairpin Conformation

Michael Wallin, Maria Ekström, and Henrik Garoff*

Department of Biosciences at Novum, Karolinska Institute, S-141 57 Huddinge, Sweden

Received 23 February 2006/ Accepted 14 July 2006

A central feature of the prevailing model for retrovirus fusion is conversion of the transmembrane (TM) subunit from a prehairpin to a hairpin-like structure. The fusion inhibition of many retroviruses, except murine leukemia virus (MLV), with peptides corresponding to interacting regions in the hairpin supports the model. MLV fusion is controlled by isomerization of the intersubunit disulfide in Env. We show here that TM peptides bind to MLV Env that has been arrested at an intermediate stage of activation by alkylation of the isomerization-active thiol in the surface subunit. This inhibits fusion rescue by dithiothreitol-mediated reduction of the surface protein-TM disulfide.

* Corresponding author. Mailing address: Department of Biosciences at Novum, Karolinska Institute, S-141 57 Huddinge, Sweden. Phone: 46-8-6089125. Fax: 46-8-7745538. E-mail: henrik.garoff{at}cbt.ki.se.

Journal of Virology, October 2006, p. 9921-9925, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.00380-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Loving, R., Li, K., Wallin, M., Sjoberg, M., Garoff, H. (2008). R-Peptide Cleavage Potentiates Fusion-Controlling Isomerization of the Intersubunit Disulfide in Moloney Murine Leukemia Virus Env. J. Virol. 82: 2594-2597 [Abstract] [Full Text]  
  • Sjoberg, M., Lindqvist, B., Garoff, H. (2008). Stabilization of TM Trimer Interactions during Activation of Moloney Murine Leukemia Virus Env. J. Virol. 82: 2358-2366 [Abstract] [Full Text]  


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