This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arumugaswami, V.
Right arrow Articles by Sun, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arumugaswami, V.
Right arrow Articles by Sun, R.

 Previous Article  |  Next Article 

Journal of Virology, October 2006, p. 9730-9740, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.00246-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

ORF18 Is a Transfactor That Is Essential for Late Gene Transcription of a Gammaherpesvirus

Vaithilingaraja Arumugaswami,1 Ting-Ting Wu,1 DeeAnn Martinez-Guzman,2 Qingmei Jia,1 Hongyu Deng,3,7 Nichole Reyes,1 and Ren Sun1,3,4,5,6*

Department of Molecular and Medical Pharmacology, David Geffen School of Medicine,1 UCLA Molecular Biology Interdepartmental Ph.D. Program,2 UCLA School of Dentistry,3 AIDS Institute,4 Jonsson Comprehensive Cancer Center,5 California Nano Systems Institute, University of California, Los Angeles, California 90095,6 Center for Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China7

Received 2 February 2006/ Accepted 11 July 2006

Lytic replication of the tumor-associated human gammaherpesviruses Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus has important implications in pathogenesis and tumorigenesis. Herpesvirus lytic genes have been temporally classified as exhibiting immediate-early (IE), early, and late expression kinetics. Though the regulation of IE and early gene expression has been studied extensively, very little is known regarding the regulation of late gene expression. Late genes, which primarily encode virion structural proteins, require viral DNA replication for their expression. We have identified a murine gammaherpesvirus 68 (MHV-68) early lytic gene, ORF18, essential for viral replication. ORF18 is conserved in both beta- and gammaherpesviruses. By generating an MHV-68 ORF18-null virus, we characterized the stage of the virus lytic cascade that requires the function of ORF18. Gene expression profiling and quantitation of viral DNA synthesis of the ORF18-null virus revealed that the expression of early genes and viral DNA replication were not affected; however, the transcription of late genes was abolished. Hence, we have identified a gammaherpesvirus-encoded factor essential for the expression of late genes independently of viral DNA synthesis.

* Corresponding author. Mailing address: Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, 23-120 Center for Health Sciences, Los Angeles, CA 90095-1735. Phone: (310) 794-5557. Fax: (310) 825-6267. E-mail: rsun{at}mednet.ucla.edu.

Journal of Virology, October 2006, p. 9730-9740, Vol. 80, No. 19
0022-538X/06/$08.00+0     doi:10.1128/JVI.00246-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guo, H., Wang, L., Peng, L., Zhou, Z. H., Deng, H. (2009). Open Reading Frame 33 of a Gammaherpesvirus Encodes a Tegument Protein Essential for Virion Morphogenesis and Egress. J. Virol. 83: 10582-10595 [Abstract] [Full Text]  
  • Wu, T.-T., Park, T., Kim, H., Tran, T., Tong, L., Martinez-Guzman, D., Reyes, N., Deng, H., Sun, R. (2009). ORF30 and ORF34 Are Essential for Expression of Late Genes in Murine Gammaherpesvirus 68. J. Virol. 83: 2265-2273 [Abstract] [Full Text]  
  • Arumugaswami, V., Sitapara, R., Hwang, S., Song, M. J., Ho, T. N., Su, N. Q., Sue, E. Y., Kanagavel, V., Xing, F., Zhang, X., Zhao, M., Deng, H., Wu, T.-T., Kanagavel, S., Zhang, L., Dandekar, S., Papp, J., Sun, R. (2009). High-Resolution Functional Profiling of a Gammaherpesvirus RTA Locus in the Context of the Viral Genome. J. Virol. 83: 1811-1822 [Abstract] [Full Text]  
  • Wong, E., Wu, T.-T., Reyes, N., Deng, H., Sun, R. (2007). Murine Gammaherpesvirus 68 Open Reading Frame 24 Is Required for Late Gene Expression after DNA Replication. J. Virol. 81: 6761-6764 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»