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Journal of Virology, September 2006, p. 8820-8823, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00719-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Integration Site Choice of a Feline Immunodeficiency Virus Vector

Yubin Kang,1,# Christopher J. Moressi,2,# Todd E. Scheetz,3,4,5 Litao Xie,1 Diane Thi Tran,1 Thomas L. Casavant,2,4,5 Prashanth Ak,6 Craig J. Benham,6 Beverly L. Davidson,7 and Paul B. McCray Jr.1*

Program in Gene Therapy, Department of Pediatrics,1 Department of Electrical and Computer Engineering,2 Department of Ophthalmology,3 Department of Biomedical Engineering,4 Center for Bioinformatics and Computational Biology,5 Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242,7 Genome Center, University of California, Davis, California 95616-88166

Received 9 April 2006/ Accepted 30 May 2006

We mapped 226 unique integration sites in human hepatoma cells following gene transfer with a feline immunodeficiency virus (FIV)-based lentivirus vector. FIV integrated across the entire length of the transcriptional units. Microarray data indicated that FIV integration favored actively transcribed genes. Approximately 21% of FIV integrations within transcriptional units occurred in genes regulated by the LEDGF/p75 transcriptional coactivator. DNA in regions of FIV insertion sites exhibited a "bendable" structure and a pattern of duplex destabilization favoring strand separation. FIV integration preferences are more similar to those of primate lentiviruses and distinct from those of Moloney murine leukemia virus, avian sarcoma leukosis virus, and foamy virus.

* Corresponding author. Mailing address: Department of Pediatrics, 240G EMRB, University of Iowa College of Medicine, Iowa City, IA 52242. Phone: (319) 335-6844. Fax: (319) 335-6925. E-mail: paul-mccray{at}uiowa.edu.

# These authors contributed equally to this work.

Journal of Virology, September 2006, p. 8820-8823, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00719-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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