This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iankov, I. D.
Right arrow Articles by Russell, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iankov, I. D.
Right arrow Articles by Russell, S. J.

 Previous Article  |  Next Article 

Journal of Virology, September 2006, p. 8530-8540, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00593-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Immunoglobulin G Antibody-Mediated Enhancement of Measles Virus Infection Can Bypass the Protective Antiviral Immune Response

Ianko D. Iankov,{dagger}* Manoj Pandey,{dagger} Mary Harvey, Guy E. Griesmann, Mark J. Federspiel, and Stephen J. Russell

Molecular Medicine Program, Mayo Clinic, Rochester, Minnesota 55905

Received 17 March 2006/ Accepted 15 June 2006

Antibodies to viral surface glycoproteins play a crucial role in immunity to measles by blocking both virus attachment and subsequent fusion with the host cell membrane. Here, we demonstrate that certain immunoglobulin G (IgG) antibodies can also enhance the entry of measles virus (MV) into monocytes and macrophages. Antibody-dependent enhancement of infectivity was observed in mouse and human macrophages using virions opsonized by a murine monoclonal antibody against the MV hemagglutinin (H) glycoprotein, polyclonal mouse anti-MV IgG, or diluted measles-immune human sera. Neither H-specific Fab fragments nor H-specific IgM could enhance MV entry in monocytes or macrophages, indicating involvement of a Fc {gamma} receptor (Fc{gamma}R)-mediated mechanism. Preincubation with an anti-fusion protein (anti-F) monoclonal antibody or a fusion-inhibitory peptide blocked infection, indicating that a functional F protein was required for viral internalization. Classical complement pathway activation did not promote infection through complement receptors and inhibited anti-H IgG-mediated enhancement. In vivo, antibody-enhanced infection allowed MV to overcome a highly protective systemic immune response in preimmunized IfnarKo-Ge46 transgenic mice. These data demonstrate a previously unidentified mechanism that may contribute to morbillivirus pathogenesis where H-specific IgG antibodies promote the spread of MV infection among Fc{gamma}R-expressing host cells. The findings point to a new model for the pathogenesis of atypical MV infection observed after immunization with formalin-inactivated MV vaccine and underscore the importance of the anti-F response after vaccination.

* Corresponding author. Mailing address: Molecular Medicine Program, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Phone: (507) 266-0385. Fax: (507) 284-8388. E-mail: Iankov.Ianko{at}mayo.edu.

{dagger} The first two authors contributed equally to this work.

Journal of Virology, September 2006, p. 8530-8540, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00593-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Martina, B. E. E., Koraka, P., Osterhaus, A. D. M. E. (2009). Dengue Virus Pathogenesis: an Integrated View. Clin. Microbiol. Rev. 22: 564-581 [Abstract] [Full Text]  
  • Yamanaka, A., Kosugi, S., Konishi, E. (2008). Infection-Enhancing and -Neutralizing Activities of Mouse Monoclonal Antibodies against Dengue Type 2 and 4 Viruses Are Controlled by Complement Levels. J. Virol. 82: 927-937 [Abstract] [Full Text]  
  • Fulci, G., Dmitrieva, N., Gianni, D., Fontana, E. J., Pan, X., Lu, Y., Kaufman, C. S., Kaur, B., Lawler, S. E., Lee, R. J., Marsh, C. B., Brat, D. J., van Rooijen, N., Rachamimov, A. S., Hochberg, F. H., Weissleder, R., Martuza, R. L., Chiocca, E. A. (2007). Depletion of Peripheral Macrophages and Brain Microglia Increases Brain Tumor Titers of Oncolytic Viruses. Cancer Res. 67: 9398-9406 [Abstract] [Full Text]  
  • Cassone, A., De Bernardis, F., Santoni, G. (2007). Anticandidal Immunity and Vaginitis: Novel Opportunities for Immune Intervention. Infect. Immun. 75: 4675-4686 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»