This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ali, A.
Right arrow Articles by Roossinck, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ali, A.
Right arrow Articles by Roossinck, M. J.

 Previous Article  |  Next Article 

Journal of Virology, September 2006, p. 8345-8350, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00568-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Analysis of Genetic Bottlenecks during Horizontal Transmission of Cucumber Mosaic Virus

Akhtar Ali,1 Hongye Li,1,{dagger} William L. Schneider,2 Diana J. Sherman,2 Stewart Gray,3 Dawn Smith,3 and Marilyn J. Roossinck1*

Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401,1 USDA-ARS Foreign Disease Weed Science Research Unit, Fort Detrick, Maryland 21702,2 USDA-ARS and Department of Plant Pathology, Cornell University, Ithaca, New York 148533

Received 17 March 2006/ Accepted 15 June 2006

Genetic bottlenecks may occur in virus populations when only a few individuals are transferred horizontally from one host to another, or when a viral population moves systemically from the infection site. Genetic bottlenecks during the systemic movement of an RNA plant virus population were reported previously (H. Li and M. J. Roossinck, J. Virol. 78:10582-10587, 2004). In this study we mechanically inoculated an artificial population consisting of 12 restriction enzyme marker mutants of Cucumber mosaic virus (CMV) onto young leaves of squash plants and used two aphid species, Aphis gossypii and Myzus persicae, to transmit the virus populations from infected source plants to healthy squash plants. Horizontal transmission by aphids constituted a significant bottleneck, as the population in the aphid-inoculated plants contained far fewer mutants than the original inoculum source. Additional experiments demonstrated that genetic variation in the artificial population of CMV is not reduced during the acquisition of the virus but is significantly reduced during the inoculation period.

* Corresponding author. Mailing address: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK 73401. Phone: (580) 224-6600. Fax: (580) 224-6692. E-mail: mroossinck{at}noble.org.

{dagger} Present address: Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310029, People's Republic of China.

Journal of Virology, September 2006, p. 8345-8350, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00568-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Grigoras, I., Timchenko, T., Katul, L., Grande-Perez, A., Vetten, H.-J., Gronenborn, B. (2009). Reconstitution of Authentic Nanovirus from Multiple Cloned DNAs. J. Virol. 83: 10778-10787 [Abstract] [Full Text]  
  • Escarmis, C., Perales, C., Domingo, E. (2009). Biological Effect of Muller's Ratchet: Distant Capsid Site Can Affect Picornavirus Protein Processing. J. Virol. 83: 6748-6756 [Abstract] [Full Text]  
  • Sanjuan, R., Agudelo-Romero, P., Elena, S. F. (2009). Upper-limit mutation rate estimation for a plant RNA virus. Biol Lett 5: 394-396 [Abstract] [Full Text]  
  • Zwart, M. P., Hemerik, L., Cory, J. S., de Visser, J. A. G.M., Bianchi, F. J.J.A., Van Oers, M. M., Vlak, J. M., Hoekstra, R. F., Van der Werf, W. (2009). An experimental test of the independent action hypothesis in virus-insect pathosystems. Proc R Soc B 276: 2233-2242 [Abstract] [Full Text]  
  • Betancourt, M., Fereres, A., Fraile, A., Garcia-Arenal, F. (2008). Estimation of the Effective Number of Founders That Initiate an Infection after Aphid Transmission of a Multipartite Plant Virus. J. Virol. 82: 12416-12421 [Abstract] [Full Text]  
  • Sanz-Ramos, M., Diaz-San Segundo, F., Escarmis, C., Domingo, E., Sevilla, N. (2008). Hidden Virulence Determinants in a Viral Quasispecies In Vivo. J. Virol. 82: 10465-10476 [Abstract] [Full Text]  
  • Simmons, H. E., Holmes, E. C., Stephenson, A. G. (2008). Rapid evolutionary dynamics of zucchini yellow mosaic virus. J. Gen. Virol. 89: 1081-1085 [Abstract] [Full Text]  
  • Turturo, C., Friscina, A., Gaubert, S., Jacquemond, M., Thompson, J. R., Tepfer, M. (2008). Evaluation of potential risks associated with recombination in transgenic plants expressing viral sequences. J. Gen. Virol. 89: 327-335 [Abstract] [Full Text]  
  • Moury, B., Fabre, F., Senoussi, R. (2007). Estimation of the number of virus particles transmitted by an insect vector. Proc. Natl. Acad. Sci. USA 104: 17891-17896 [Abstract] [Full Text]  
  • Dietrich, C., Al Abdallah, Q., Lintl, L., Pietruszka, A., Maiss, E. (2007). A chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection. J. Gen. Virol. 88: 2846-2851 [Abstract] [Full Text]  
  • de la Iglesia, F., Elena, S. F. (2007). Fitness Declines in Tobacco Etch Virus upon Serial Bottleneck Transfers. J. Virol. 81: 4941-4947 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»