This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ashby, J.
Right arrow Articles by Heinlein, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ashby, J.
Right arrow Articles by Heinlein, M.

 Previous Article  |  Next Article 

Journal of Virology, September 2006, p. 8329-8344, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00540-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Tobacco Mosaic Virus Movement Protein Functions as a Structural Microtubule-Associated Protein

Jamie Ashby,2,3,{dagger} Emmanuel Boutant,1,{dagger} Mark Seemanpillai,1,{dagger} Adrian Sambade,1 Christophe Ritzenthaler,1 and Manfred Heinlein1,2,3*

Institut de Biologie Moléculaire des Plantes, 12 Rue du Général Zimmer, 67084 Strasbourg, France,1 Botanisches Institut der Universität Basel, Hebelstrasse 1, 4056 Basel, Switzerland,2 Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland3

Received 15 March 2006/ Accepted 4 June 2006

The cell-to-cell spread of Tobacco mosaic virus infection depends on virus-encoded movement protein (MP), which is believed to form a ribonucleoprotein complex with viral RNA (vRNA) and to participate in the intercellular spread of infectious particles through plasmodesmata. Previous studies in our laboratory have provided evidence that the vRNA movement process is correlated with the ability of the MP to interact with microtubules, although the exact role of this interaction during infection is not known. Here, we have used a variety of in vivo and in vitro assays to determine that the MP functions as a genuine microtubule-associated protein that binds microtubules directly and modulates microtubule stability. We demonstrate that, unlike MP in whole-cell extract, microtubule-associated MP is not ubiquitinated, which strongly argues against the hypothesis that microtubules target the MP for degradation. In addition, we found that MP interferes with kinesin motor activity in vitro, suggesting that microtubule-associated MP may interfere with kinesin-driven transport processes during infection.

* Corresponding author. Mailing address: Institut de Biologie Moléculaire des Plantes, 12, rue du Général Zimmer, 67084 Strasbourg, France. Phone: 33 3 88 417 258. Fax: 33 3 88 614 442. E-mail: manfred.heinlein{at}ibmp-ulp.u-strasbg.fr.

{dagger} Contributed equally to this work.

Journal of Virology, September 2006, p. 8329-8344, Vol. 80, No. 17
0022-538X/06/$08.00+0     doi:10.1128/JVI.00540-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stonebloom, S., Burch-Smith, T., Kim, I., Meinke, D., Mindrinos, M., Zambryski, P. (2009). Loss of the plant DEAD-box protein ISE1 leads to defective mitochondria and increased cell-to-cell transport via plasmodesmata. Proc. Natl. Acad. Sci. USA 106: 17229-17234 [Abstract] [Full Text]  
  • Hofmann, C., Niehl, A., Sambade, A., Steinmetz, A., Heinlein, M. (2009). Inhibition of Tobacco Mosaic Virus Movement by Expression of an Actin-Binding Protein. Plant Physiol. 149: 1810-1823 [Abstract] [Full Text]  
  • Ruggenthaler, P., Fichtenbauer, D., Krasensky, J., Jonak, C., Waigmann, E. (2009). Microtubule-Associated Protein AtMPB2C Plays a Role in Organization of Cortical Microtubules, Stomata Patterning, and Tobamovirus Infectivity. Plant Physiol. 149: 1354-1365 [Abstract] [Full Text]  
  • Harries, P. A., Palanichelvam, K., Yu, W., Schoelz, J. E., Nelson, R. S. (2009). The Cauliflower Mosaic Virus Protein P6 Forms Motile Inclusions That Traffic along Actin Microfilaments and Stabilize Microtubules. Plant Physiol. 149: 1005-1016 [Abstract] [Full Text]  
  • Xiong, R., Wu, J., Zhou, Y., Zhou, X. (2008). Identification of a Movement Protein of the Tenuivirus Rice Stripe Virus. J. Virol. 82: 12304-12311 [Abstract] [Full Text]  
  • Brandner, K., Sambade, A., Boutant, E., Didier, P., Mely, Y., Ritzenthaler, C., Heinlein, M. (2008). Tobacco Mosaic Virus Movement Protein Interacts with Green Fluorescent Protein-Tagged Microtubule End-Binding Protein 1. Plant Physiol. 147: 611-623 [Abstract] [Full Text]  
  • Schepetilnikov, M. V., Solovyev, A. G., Gorshkova, E. N., Schiemann, J., Prokhnevsky, A. I., Dolja, V. V., Morozov, S. Y. (2008). Intracellular Targeting of a Hordeiviral Membrane-Spanning Movement Protein: Sequence Requirements and Involvement of an Unconventional Mechanism. J. Virol. 82: 1284-1293 [Abstract] [Full Text]  
  • Curin, M., Ojangu, E.-L., Trutnyeva, K., Ilau, B., Truve, E., Waigmann, E. (2007). MPB2C, a Microtubule-Associated Plant Factor, Is Required for Microtubular Accumulation of Tobacco Mosaic Virus Movement Protein in Plants. Plant Physiol. 143: 801-811 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»