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Journal of Virology, August 2006, p. 7902-7908, Vol. 80, No. 16
0022-538X/06/$08.00+0     doi:10.1128/JVI.00483-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Dodecamer Structure of Severe Acute Respiratory Syndrome Coronavirus Nonstructural Protein nsp10

Dan Su,1,2,{dagger} Zhiyong Lou,1,2,{dagger} Fei Sun,1,2 Yujia Zhai,1,2 Haitao Yang,1,2 Rongguang Zhang,2,3 Andrzej Joachimiak,3 Xuejun C. Zhang,2,4 Mark Bartlam,1,2 and Zihe Rao1,2*

"Tsinghua-IBP Joint Research Group for Structural Biology," Tsinghua University, Beijing 100084, China,1 National Laboratory of Biomacromolecules, Institute of Biophysics (IBP), Chinese Academy of Sciences, Beijing 100101, China,2 Biosciences Division, Argonne National Laboratory, Argonne, Illinois 60439,3 Crystallography Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 731044

Received 8 March 2006/ Accepted 9 May 2006

The severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural proteins nsp1 to nsp16 have been implicated by genetic analysis in the assembly of a functional replication/transcription complex. We report the crystal structure of nsp10 from SARS-CoV at 2.1-Å resolution. The nsp10 structure has a novel fold, and 12 identical subunits assemble to form a unique spherical dodecameric architecture. Two zinc fingers have been identified from the nsp10 monomer structure with the sequence motifs C-(X)2-C-(X)5-H-(X)6-C and C-(X)2-C-(X)7-C-(X)-C. The nsp10 crystal structure is the first of a new class of zinc finger protein three-dimensional structures to be revealed experimentally. The zinc finger sequence motifs are conserved among all three coronavirus antigenic groups, implicating an essential function for nsp10 in all coronaviruses. Based on the structure, we propose that nsp10 is a transcription factor for coronavirus replication/transcription.

* Corresponding author. Mailing address: Laboratory of Structural Biology, Life Sciences Building, Tsinghua University, Beijing 100084, China. Phone: 86 10 62771493. Fax: 86 10 62773145. E-mail: raozh{at}xtal.tsinghua.edu.cn.

{dagger} These authors made equal contributions.

Journal of Virology, August 2006, p. 7902-7908, Vol. 80, No. 16
0022-538X/06/$08.00+0     doi:10.1128/JVI.00483-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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