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Journal of Virology, August 2006, p. 7740-7743, Vol. 80, No. 15
0022-538X/06/$08.00+0     doi:10.1128/JVI.00436-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Compensatory Capsid Protein Mutations in Cucumber Mosaic Virus Confer Systemic Infectivity in Squash (Cucurbita pepo)

Jeremy R. Thompson,1,2 Stephanie Doun,1 and Keith L. Perry1*

Department of Plant Pathology, 334 Plant Science Building, Cornell University, Ithaca, New York 14850,1 ICGEB, Biosafety Outstation, Via Piovega 23, 31050 Ca'Tron di Roncade, Italy2

Received 1 March 2006/ Accepted 9 May 2006

Cucumber mosaic virus (CMV) systemically infects both tobacco and zucchini squash. CMV capsid protein loop mutants with single-amino-acid substitutions are unable to systemically infect squash, but they revert to a wild-type phenotype in the presence of an additional, specific single-site substitution. The D118A, T120A, D192A, and D197A loop mutants reverted to a wild-type phenotype but did so in combination with P56S, P77L, A162V, and I53F or T124I mutations, respectively. The possible effect of these compensatory mutations on other, nonsystemically infecting loop mutants was tested with the F117A mutant and found to be neutral, thus indicating a specificity to the observed changes.

* Corresponding author. Mailing address: Department of Plant Pathology, 334 Plant Science Building, Cornell University, Ithaca, NY 14850. Phone: (607) 255-9744. Fax: (607) 255-4471. E-mail: KLP3{at}cornell.edu.

Journal of Virology, August 2006, p. 7740-7743, Vol. 80, No. 15
0022-538X/06/$08.00+0     doi:10.1128/JVI.00436-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Luna, E., Rodriguez-Huete, A., Rincon, V., Mateo, R., Mateu, M. G. (2009). Systematic Study of the Genetic Response of a Variable Virus to the Introduction of Deleterious Mutations in a Functional Capsid Region. J. Virol. 83: 10140-10151 [Abstract] [Full Text]  
  • Mateo, R., Mateu, M. G. (2007). Deterministic, Compensatory Mutational Events in the Capsid of Foot-and-Mouth Disease Virus in Response to the Introduction of Mutations Found in Viruses from Persistent Infections. J. Virol. 81: 1879-1887 [Abstract] [Full Text]  


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