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Journal of Virology, July 2006, p. 6952-6963, Vol. 80, No. 14
0022-538X/06/$08.00+0     doi:10.1128/JVI.00187-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Sequence Analysis and Organization of the Neodiprion abietis Nucleopolyhedrovirus Genome

Simon P. Duffy,1 Aaron M. Young,1 Benoit Morin,2 Christopher J. Lucarotti,2 Ben F. Koop,1 and David B. Levin1*

Department of Biology, University of Victoria, Victoria, British Columbia, Canada V8W 2Y2,1 Canadian Forest Service, Atlantic Forestry Centre, Fredericton, New Brunswick, Canada E3B 5P72

Received 26 January 2006/ Accepted 27 April 2006

Of 30 baculovirus genomes that have been sequenced to date, the only nonlepidopteran baculoviruses include the dipteran Culex nigripalpus nucleopolyhedrovirus and two hymenopteran nucleopolyhedroviruses that infect the sawflies Neodiprion lecontei (NeleNPV) and Neodiprion sertifer (NeseNPV). This study provides a complete sequence and genome analysis of the nucleopolyhedrovirus that infects the balsam fir sawfly Neodiprion abietis (Hymenoptera, Symphyta, Diprionidae). The N. abietis nucleopolyhedrovirus (NeabNPV) is 84,264 bp in size, with a G+C content of 33.5%, and contains 93 predicted open reading frames (ORFs). Eleven predicted ORFs are unique to this baculovirus, 10 ORFs have a putative sequence homologue in the NeleNPV genome but not the NeseNPV genome, and 1 ORF (neab53) has a putative sequence homologue in the NeseNPV genome but not the NeleNPV genome. Specific repeat sequences are coincident with major genome rearrangements that distinguish NeabNPV and NeleNPV. Genes associated with these repeat regions encode a common amino acid motif, suggesting that they are a family of repeated contiguous gene clusters. Lepidopteran baculoviruses, similarly, have a family of repeated genes called the bro gene family. However, there is no significant sequence similarity between the NeabNPV and bro genes. Homologues of early-expressed genes such as ie-1 and lef-3 were absent in NeabNPV, as they are in the previously sequenced hymenopteran baculoviruses. Analyses of ORF upstream sequences identified potential temporally distinct genes on the basis of putative promoter elements.

* Corresponding author. Mailing address: Department of Biology, University of Victoria, Victoria, BC, Canada V8W 2Y2. Phone: (250) 472-4069. Fax: (250) 472-4075. E-mail: dlevin{at}uvic.ca.

Journal of Virology, July 2006, p. 6952-6963, Vol. 80, No. 14
0022-538X/06/$08.00+0     doi:10.1128/JVI.00187-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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