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Journal of Virology, July 2006, p. 6697-6701, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.02388-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A Highly Restricted T-Cell Receptor Dominates the CD8+ T-Cell Response to Parvovirus B19 Infection in HLA-A*2402-Positive Individuals

V. Kasprowicz,1,4* A. Isa,2 K. Jeffery,3 K. Broliden,2 T. Tolfvenstam,2 P. Klenerman,4 and P. Bowness1

MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, Oxford, United Kingdom,1 Division of Clinical Virology, Karolinska Institute, Stockholm, Sweden,2 Department of Virology, John Radcliffe Hospital, Oxford, United Kingdom,3 Peter Medawar Building for Pathogen Research, Nuffield Department of Medicine, Oxford University, Oxford, United Kingdom4

Received 14 November 2005/ Accepted 15 March 2006

Six of seven HLA-A*2402-positive individuals with acute parvovirus B19 infections made vigorous CD8-positive cytotoxic T-cell (CTL) responses to the viral epitope FYTPLADQF. All responders showed highly focused T-cell receptor (TCR) usage, using almost exclusively BV5.1. The BV5.1 TCR dominated the acute response, was maintained over time, and was also used by a remotely infected individual. Nine CTL clones and two oligoclonal lines obtained from three unrelated individuals used BV5.1, BJ2.1, and a conserved TCR CDR3 of nine amino acids. This commonly recognized epitope is likely important in long-term protective immunity and should be included in vaccine design.

* Corresponding author. Present address: Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, 6th Floor, Charlestown, MA 02129. Phone: (617) 959-2319. Fax: (617) 724-5411. E-mail: vkasprowicz{at}partners.org.

Journal of Virology, July 2006, p. 6697-6701, Vol. 80, No. 13
0022-538X/06/$08.00+0     doi:10.1128/JVI.02388-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Venturi, V., Chin, H. Y., Price, D. A., Douek, D. C., Davenport, M. P. (2008). The Role of Production Frequency in the Sharing of Simian Immunodeficiency Virus-Specific CD8+ TCRs between Macaques. J. Immunol. 181: 2597-2609 [Abstract] [Full Text]  


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