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Journal of Virology, June 2006, p. 5919-5926, Vol. 80, No. 12
0022-538X/06/$08.00+0     doi:10.1128/JVI.00046-06

A Bicistronic Subgenomic mRNA Encodes both the ORF2 and ORF3 Proteins of Hepatitis E Virus

Judith Graff,* Udana Torian, Hanh Nguyen, and Suzanne U. Emerson

Molecular Hepatitis Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892

Received 6 January 2006/ Accepted 4 April 2006

Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and a single subgenomic RNA. This subgenomic RNA, which was capped, initiated at nucleotide 5122 downstream of the first two methionine codons in ORF3 and was bicistronic; two closely spaced methionine codons in different reading frames were used for the initiation of ORF3 and ORF2 translation.

* Corresponding author. Mailing address: National Institutes of Health, NIAID, LID, Molecular Hepatitis Section, Building 50, Room 6535, 50 South Drive, MSC 8009, Bethesda, MD 20892-8009. Phone: (301) 504-2313. Fax: (301) 402-0524. E-mail: jgraff{at}niaid.nih.gov.

Journal of Virology, June 2006, p. 5919-5926, Vol. 80, No. 12
0022-538X/06/$08.00+0     doi:10.1128/JVI.00046-06




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