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Journal of Virology, June 2006, p. 5822-5832, Vol. 80, No. 12
0022-538X/06/$08.00+0     doi:10.1128/JVI.02707-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Bovine Herpesvirus 1 UL49.5 Protein Inhibits the Transporter Associated with Antigen Processing despite Complex Formation with Glycoprotein M

Andrea D. Lipinska,1,2 Danijela Koppers-Lalic,1 Michal Rychlowski,2 Pieter Admiraal,1 Frans A. M. Rijsewijk,3 Krystyna Bienkowska-Szewczyk,2 and Emmanuel J. H. J. Wiertz1*

Department of Medical Microbiology, Leiden University Medical Center, 2300 RC Leiden,1 Virus Discovery Unit, Animal Sciences Group, 8200 AB Lelystad, The Netherlands,3 Department of Molecular Virology, University of Gdansk, 80-822 Gdansk, Poland2

Received 23 December 2005/ Accepted 29 March 2006

Bovine herpesvirus 1 (BHV-1) interferes with peptide translocation by the transporter associated with antigen processing (TAP). Recently, the UL49.5 gene product of BHV-1 was identified as the protein responsible for the observed inhibition of TAP. In BHV-1-infected cells and virions, the UL49.5 protein forms a complex with glycoprotein M (gM). Hence, it was investigated whether UL49.5 can combine the interactions with gM and the TAP complex. In cell lines constitutively expressing both UL49.5 and gM, UL49.5 appears to be required for functional processing of gM. Immunofluorescence-confocal laser scanning microscopy demonstrated that both proteins are interdependent for their redistribution from the endoplasmic reticulum to the trans-Golgi network. Remarkably, expression of cloned gM results in the abrogation of the UL49.5-mediated inhibition of TAP and prevents the degradation of the transporter. However, in BHV-1-infected cells, differences in UL49.5 and gM expression kinetics were seen to create a window of opportunity at the early stages of infection, during which time the UL49.5 protein can act on TAP without gM interference. Moreover, in later periods, non-gM-associated UL49.5 can be detected in addition to the UL49.5/gM complex. Thus, it has been deduced that different functions of UL49.5, editing of gM processing and inhibition of TAP, can be combined during BHV-1 infection.

* Corresponding author. Mailing address: Department of Medical Microbiology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: 31-71-526-3932. Fax: 31-71-524-8148. E-mail: wiertz{at}lumc.nl.

Journal of Virology, June 2006, p. 5822-5832, Vol. 80, No. 12
0022-538X/06/$08.00+0     doi:10.1128/JVI.02707-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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