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Journal of Virology, June 2006, p. 5482-5493, Vol. 80, No. 11
0022-538X/06/$08.00+0     doi:10.1128/JVI.02735-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Expression Profiles of Bovine Adeno-Associated Virus and Avian Adeno-Associated Virus Display Significant Similarity to That of Adeno-Associated Virus Type 5

Jianming Qiu, Fang Cheng, and David J. Pintel*

Department of Molecular Microbiology and Immunology, Life Sciences Center, University of Missouri—Columbia, Columbia, Missouri 65211

Received 29 December 2005/ Accepted 9 March 2006

We present the first detailed expression profiles of nonprimate-derived adeno-associated viruses, namely, bovine adeno-associated virus (B-AAV) and avian adeno-associated virus (A-AAV), which were obtained after the infection of cell lines derived from their natural hosts. In general, the profiles of B-AAV and A-AAV were quite similar to that of AAV5; however, both exhibited features found for AAV2 as well. Like adeno-associated virus type 5 (AAV5), B-AAV and A-AAV utilized an internal polyadenylation site [(pA)p]; however, it was used to greater relative levels by B-AAV than by A-AAV. Similar to AAV5, >99% of B-AAV RNAs generated from upstream promoters were polyadenylated at (pA)p and hence not spliced. In contrast, ca. 50% of the A-AAV RNAs generated from upstream promoters read through (pA)p, as seen for AAV2. However, A-AAV generated lower levels of spliced P5 and P19 products than does AAV2, suggesting that A-AAV generates lower relative levels of Rep 68 and Rep 40. An additional difference in the expression profile of these viruses was that B-AAV generated a greater level of ITR-initiated RNAs than did A-AAV or AAV5. In addition, we demonstrate that, like AAV2, transactivation of transcription of the capsid-gene promoter of B-AAV required both adenovirus and targeting of its Rep protein to the transcription template; however, expression of the capsid-gene promoter of A-AAV was, like AAV5, largely independent of both adenovirus and its Rep proteins.

* Corresponding author. Mailing address: Life Sciences Center, 1201 Rollins Rd., University of Missouri-Columbia, Columbia, MO 65211. Phone: (573) 882-3920. Fax: (573) 882-9676. E-mail: pinteld{at}missouri.edu.

Journal of Virology, June 2006, p. 5482-5493, Vol. 80, No. 11
0022-538X/06/$08.00+0     doi:10.1128/JVI.02735-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Qiu, J., Cheng, F., Johnson, F. B., Pintel, D. (2007). The Transcription Profile of the Bocavirus Bovine Parvovirus Is Unlike Those of Previously Characterized Parvoviruses. J. Virol. 81: 12080-12085 [Abstract] [Full Text]  
  • Ye, C., Pintel, D. J. (2007). Upstream AP1- and CREB-Binding Sites Confer High Basal Activity on the Adeno-Associated Virus Type 5 Capsid Gene Promoter. J. Virol. 81: 2605-2613 [Abstract] [Full Text]  


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