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Journal of Virology, May 2006, p. 5082-5085, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.5082-5085.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of Novel Adeno-Associated Virus Isolates in ATCC Virus Stocks

Michael Schmidt,1 Emmanuelle Grot,1 Peter Cervenka,1 Sandra Wainer,1 Charles Buck,2 and John A. Chiorini1*

Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892,1 Department of Virology, American Type Culture Collection (ATCC), Manassas, Virginia 201082

Received 19 October 2005/ Accepted 20 February 2006

Adeno-associated viruses (AAVs) depend on a helper virus for efficient replication. To identify novel AAV isolates, we screened a diverse set of virus isolates for the presence of AAV DNA. AAVs found in 10 simian adenovirus isolates showed greater than 96% homology to AAV1 and AAV6 but had distinct biological properties. Two representatives of this group, AAV(VR-195) and AAV(VR-355), were studied in more detail. While the novel AAVs had high sequence homologies and required sialic acid for cell binding and transduction, differences were observed in lectin competition, resulting in distinct tropisms in human cancer cell lines.

* Corresponding author. Mailing address: NIH 10/1N113, 10 Center Dr., MSC1190, Bethesda, MD 20892. Phone: (301) 496-4279. Fax: (301) 402-1228. E-mail: jchiorini{at}dir.nidcr.nih.gov.

Journal of Virology, May 2006, p. 5082-5085, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.5082-5085.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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