This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ceccaldi, P.-E.
Right arrow Articles by Ozden, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ceccaldi, P.-E.
Right arrow Articles by Ozden, S.

 Previous Article  |  Next Article 

Journal of Virology, May 2006, p. 4771-4780, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.4771-4780.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

DC-SIGN Facilitates Fusion of Dendritic Cells with Human T-Cell Leukemia Virus Type 1-Infected Cells

Pierre-Emmanuel Ceccaldi,1 Frédéric Delebecque,2 Marie-Christine Prevost,3 Arnaud Moris,2 Jean-Pierre Abastado,4,{dagger} Antoine Gessain,1 Olivier Schwartz,2 and Simona Ozden1*

Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur, Paris, France,1 Groupe Virus et Immunité, Institut Pasteur, Paris, France,2 Plate-Forme Microscopie Electronique, Institut Pasteur, Paris, France,3 Immuno Designed Molecules, Paris, France4

Received 8 December 2005/ Accepted 16 February 2006

Interactions between the oncogenic retrovirus human T-cell leukemia virus type 1 (HTLV-1) and dendritic cells (DCs) are poorly characterized. We show here that monocyte-derived DCs form syncytia and are infected upon coculture with HTLV-1-infected lymphocytes. We examined the role of DC-specific ICAM-3-grabbing nonintegrin (DC-SIGN), a C-type lectin expressed in DCs, in HTLV-1-induced syncytium formation. DC-SIGN is known to bind with high affinity to various viral envelope glycoproteins, including human immunodeficiency virus (HIV) and hepatitis C virus, as well as to the cellular receptors ICAM-2 and ICAM-3. After cocultivating DCs and HTLV-1-infected cells, we found that anti-DC-SIGN monoclonal antibodies (MAbs) were able to decrease the number and size of HTLV-1-induced syncytia. Moreover, expression of the lectin in epithelial-cell lines dramatically enhanced the ability to fuse with HTLV-1-positive cells. Interestingly, in contrast to the envelope (Env) glycoproteins of HIV and other viruses, that of HTLV-1 does not bind directly to DC-SIGN. The facilitating role of the lectin in HTLV-1 syncytium formation is mediated by its interaction with ICAM-2 and ICAM-3, as demonstrated by use of MAbs directed against these adhesion molecules. Altogether, our results indicate that DC-SIGN facilitates HTLV-1 infection and fusion of DCs through an ICAM-dependent mechanism.

* Corresponding author. Mailing address: Unité Epidémiologie et Physiopathologie des Virus Oncogènes, Département Ecosystèmes et Epidémiologie des Maladies Infectieuses, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 87 82. Fax: 33 1 40 61 34 65. E-mail: sozd{at}pasteur.fr.

{dagger} Present address: Departement d'Immunologie, Institut Cochin, Paris, France.

Journal of Virology, May 2006, p. 4771-4780, Vol. 80, No. 10
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.10.4771-4780.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Taylor, J. M., Brown, M., Nejmeddine, M., Kim, K. J., Ratner, L., Lairmore, M., Nicot, C. (2009). Novel Role for Interleukin-2 Receptor-Jak Signaling in Retrovirus Transmission. J. Virol. 83: 11467-11476 [Abstract] [Full Text]  
  • Manuel, S. L., Schell, T. D., Acheampong, E., Rahman, S., Khan, Z. K., Jain, P. (2009). Presentation of human T cell leukemia virus type 1 (HTLV-1) Tax protein by dendritic cells: the underlying mechanism of HTLV-1-associated neuroinflammatory disease. J. Leukoc. Biol. 86: 1205-1216 [Abstract] [Full Text]  
  • Jain, P., Manuel, S. L., Khan, Z. K., Ahuja, J., Quann, K., Wigdahl, B. (2009). DC-SIGN Mediates Cell-Free Infection and Transmission of Human T-Cell Lymphotropic Virus Type 1 by Dendritic Cells. J. Virol. 83: 10908-10921 [Abstract] [Full Text]  
  • Kashima, S., Rodrigues, E. S., Azevedo, R., da Cruz Castelli, E., Mendes-Junior, C. T., Yoshioka, F. K. N., da Silva, I. T., Takayanagui, O. M., Covas, D. T. (2009). DC-SIGN (CD209) gene promoter polymorphisms in a Brazilian population and their association with human T-cell lymphotropic virus type 1 infection. J. Gen. Virol. 90: 927-934 [Abstract] [Full Text]  
  • Balestrieri, E., Ascolani, A., Igarashi, Y., Oki, T., Mastino, A., Balzarini, J., Macchi, B. (2008). Inhibition of Cell-to-Cell Transmission of Human T-Cell Lymphotropic Virus Type 1 In Vitro by Carbohydrate-Binding Agents. Antimicrob. Agents Chemother. 52: 2771-2779 [Abstract] [Full Text]  
  • Chevalier, S. A., Walic, M., Calattini, S., Mallet, A., Prevost, M.-C., Gessain, A., Mahieux, R. (2007). Construction and Characterization of a Full-Length Infectious Simian T-Cell Lymphotropic Virus Type 3 Molecular Clone. J. Virol. 81: 6276-6285 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»