This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hrdlicková, R.
Right arrow Articles by Bose, H. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hrdlicková, R.
Right arrow Articles by Bose, H. R., Jr.

 Previous Article  |  Next Article 

Journal of Virology, January 2006, p. 281-295, Vol. 80, No. 1
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.1.281-295.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mechanism of Telomerase Activation by v-Rel and Its Contribution to Transformation

Radmila Hrdlicková, Jirí Nehyba, Andrew S. Liss, and Henry R. Bose Jr.*

Section of Molecular Genetics and Microbiology, School of Biological Sciences and Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas 78712-1095

Received 20 August 2005/ Accepted 6 October 2005

Telomerase is activated during the transformation of lymphoid cells and fibroblasts by v-Rel, the oncogenic member of the Rel/NF-{kappa}B family of transcription factors. v-Rel-transformed cell lines have longer telomeres than untransformed chicken lymphoid cells and have high levels of telomerase activity. v-Rel-mediated activation of telomerase is achieved by multiple mechanisms. The expression of the gene encoding the catalytic subunit of telomerase (TERT) was directly upregulated by v-Rel. Moreover, the expression of v-Rel altered the ratio of alternatively spliced and full-length TERT transcripts in favor of the full-length forms. The activation of telomerase by v-Rel in lymphocytes was also accompanied by inactivation of nuclear inhibitors. The inhibition of telomerase activity in v-Rel-transformed cell lines led to apoptosis within 24 h. The expression of v-Rel in a macrophage cell line resulted in elevated levels of reactive oxygen species (ROS), increased telomerase activity, and increased sensitivity to telomerase inhibitors. In contrast, the ectopic expression of TERT decreased the extent of apoptosis induced by ROS. The activation of telomerase by v-Rel may, therefore, partially protect the transformed cells from apoptosis induced by ROS.

* Corresponding author. Mailing address: Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, TX 78712-1095. Phone: (512) 471-5525. Fax: (512) 471-2130. E-mail: bose{at}mail.utexas.edu.

Journal of Virology, January 2006, p. 281-295, Vol. 80, No. 1
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.1.281-295.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hrdlickova, R., Nehyba, J., Bose, H. R. Jr. (2009). Regulation of Telomerase Activity by Interferon Regulatory Factors 4 and 8 in Immune Cells. Mol. Cell. Biol. 29: 929-941 [Abstract] [Full Text]  
  • Dutta, J., Fan, G., Gelinas, C. (2008). CAPER{alpha} Is a Novel Rel-TAD-Interacting Factor That Inhibits Lymphocyte Transformation by the Potent Rel/NF-{kappa}B Oncoprotein v-Rel. J. Virol. 82: 10792-10802 [Abstract] [Full Text]  
  • Gupta, N., Delrow, J., Drawid, A., Sengupta, A. M., Fan, G., Gelinas, C. (2008). Repression of B-Cell Linker (BLNK) and B-Cell Adaptor for Phosphoinositide 3-Kinase (BCAP) Is Important for Lymphocyte Transformation by Rel Proteins. Cancer Res. 68: 808-814 [Abstract] [Full Text]  
  • Yang, F., Xian, R. R., Li, Y., Polony, T. S., Beemon, K. L. (2007). Telomerase reverse transcriptase expression elevated by avian leukosis virus integration in B cell lymphomas. Proc. Natl. Acad. Sci. USA 104: 18952-18957 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»