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Journal of Virology, January 2006, p. 201-209, Vol. 80, No. 1
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.1.201-209.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Pseudorabies Virus VP1/2 Tegument Protein Is Required for Intracellular Capsid Transport{dagger}

G. W. Gant Luxton,1,{ddagger} Joy I-Hsuan Lee,1,{ddagger} Sarah Haverlock-Moyns,1 Joseph Martin Schober,2 and Gregory Allan Smith1*

Department of Microbiology-Immunology,1 Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 606112

Received 8 June 2005/ Accepted 30 September 2005

Transport of capsids in cells is critical to alphaherpesvirus infection and pathogenesis; however, viral factors required for transport have yet to be identified. Here we provide a detailed examination of capsid dynamics during the egress phase of infection in Vero cells infected with pseudorabies virus. We demonstrate that the VP1/2 tegument protein is required for processive microtubule-based transport of capsids in the cytoplasm. A second tegument protein that binds to VP1/2, UL37, was necessary for wild-type transport but was not essential for this process. Both proteins were also required for efficient nuclear egress of capsids to the cytoplasm.

* Corresponding author. Mailing address: Department of Microbiology-Immunology, Ward Bldg., Rm. 10-105, Northwestern University Feinberg School of Medicine, Chicago, IL 60611. Phone: (312) 503-3745. Fax: (312) 503-1339. E-mail: g-smith3{at}northwestern.edu.

{dagger} Supplementary material for this article may be found at http://jvi.asm.org/.

{ddagger} G.W.G.L. and J.I.L. contributed equally to this study.

Journal of Virology, January 2006, p. 201-209, Vol. 80, No. 1
0022-538X/06/$08.00+0     doi:10.1128/JVI.80.1.201-209.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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