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Journal of Virology, May 2005, p. 5857-5862, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5857-5862.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Nearby Clusters of Hemagglutinin Residues Sustain SLAM-Dependent Canine Distemper Virus Entry in Peripheral Blood Mononuclear Cells

Veronika von Messling,1,{dagger} Numan Oezguen,2 Qi Zheng,1 Sompong Vongpunsawad,1 Werner Braun,2 and Roberto Cattaneo1,3*

Molecular Medicine Program,1 Virology and Gene Therapy Graduate Track, Mayo Clinic College of Medicine, Rochester, Minnesota,3 Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, Texas2

Received 24 September 2004/ Accepted 3 December 2004

Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.

* Corresponding author. Mailing address: Molecular Medicine Program, Mayo Clinic, Guggenheim 1838, 200 1st Street SW, Rochester, MN 55905. Phone: (507) 284-0171. Fax: (507) 266-2122. E-mail: cattaneo.roberto{at}mayo.edu.

{dagger} Present address: INRS-Institut Armand-Frappier, Universite du Quebec, Quebec, Laval H7V 1B7, Canada.

Journal of Virology, May 2005, p. 5857-5862, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5857-5862.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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