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Journal of Virology, May 2005, p. 5732-5742, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5732-5742.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Homologous Crossovers among Molecules of Brome Mosaic Bromovirus RNA1 or RNA2 Segments In Vivo

Anna Urbanowicz,1 Magdalena Alejska,1 Piotr Formanowicz,1,2 Jacek Blazewicz,1 Marek Figlerowicz,1 and Jozef J. Bujarski1,3*

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland,1 Plant Molecular Biology Center and the Department of Biological Sciences, Northern Illinois University, De Kalb, Illinois 60115,3 Institute of Computing Science, Poznan University of Technology, Piotrowo 3a, 60-965 Poznan, Poland2

Received 21 August 2004/ Accepted 23 December 2004

Previously we demonstrated frequent homologous crossovers among molecules of the RNA3 segment in the tripartite brome mosaic bromovirus (BMV) RNA genome (A. Bruyere, M. Wantroba, S. Flasinski, A. Dzianott, and J. J. Bujarski, J. Virol. 74:4214-4219, 2000). To further our knowledge about mechanisms of viral RNA genome variability, in this paper we have studied homologous recombination in BMV RNA1 and RNA2 components during infection. We have found that basal RNA-RNA crossovers could occur within coding regions of both RNAs, although recombination frequencies slightly varied at different RNA sections. In all cases, the frequencies were much lower than the rate observed for the intercistronic recombination hot spot in BMV RNA3. Probability calculations accounted for at least one homologous crossover per RNA molecule per replication cycle. In addition, we have demonstrated an efficient repair of mutations within the conserved 3' and 5' noncoding regions, most likely due to error-prone BMV RNA replication. Overall, our data verify that homologous crossovers are common events a during virus life cycle, and we discuss their importance for viral RNA genetics.

* Corresponding author. Mailing address: Department of Biological Sciences, Montgomery Hall, Northern Illinois University, De Kalb, IL 60015. Phone: (815) 753-0601. Fax: (815) 753-7855. E-mail: jbujarski{at}niu.edu.

Journal of Virology, May 2005, p. 5732-5742, Vol. 79, No. 9
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.9.5732-5742.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Codoner, F. M., Elena, S. F. (2008). The promiscuous evolutionary history of the family Bromoviridae. J. Gen. Virol. 89: 1739-1747 [Abstract] [Full Text]  
  • Yi, G., Kao, C. (2008). cis- and trans-Acting Functions of Brome Mosaic Virus Protein 1a in Genomic RNA1 Replication. J. Virol. 82: 3045-3053 [Abstract] [Full Text]  
  • Wierzchoslawski, R., Urbanowicz, A., Dzianott, A., Figlerowicz, M., Bujarski, J. J. (2006). Characterization of a Novel 5' Subgenomic RNA3a Derived from RNA3 of Brome Mosaic Bromovirus. J. Virol. 80: 12357-12366 [Abstract] [Full Text]  
  • Alejska, M., Figlerowicz, M., Malinowska, N., Urbanowicz, A., Figlerowicz, M. (2005). A universal BMV-based RNA recombination system--how to search for general rules in RNA recombination. Nucleic Acids Res 33: e105-e105 [Abstract] [Full Text]  


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