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Journal of Virology, April 2005, p. 5211-5214, Vol. 79, No. 8
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.8.5211-5214.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Weak Palindromic Consensus Sequences Are a Common Feature Found at the Integration Target Sites of Many Retroviruses

Xiaolin Wu,1* Yuan Li,2 Bruce Crise,2 Shawn M. Burgess,3 and David J. Munroe1

Laboratory of Molecular Technology,1 AIDS Vaccine Program, Scientific Application International Corporation, National Cancer Institute at Frederick, Frederick,2 Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland3

Received 2 September 2004/ Accepted 14 December 2004

Integration into the host genome is one of the hallmarks of the retroviral life cycle and is catalyzed by virus-encoded integrases. While integrase has strict sequence requirements for the viral DNA ends, target site sequences have been shown to be very diverse. We carefully examined a large number of integration target site sequences from several retroviruses, including human immunodeficiency virus type 1, simian immunodeficiency virus, murine leukemia virus, and avian sarcoma-leukosis virus, and found that a statistical palindromic consensus, centered on the virus-specific duplicated target site sequence, was a common feature at integration target sites for these retroviruses.

* Corresponding author. Mailing address: Laboratory of Molecular Technology, Scientific Application International Corporation, National Cancer Institute at Frederick, 915 Toll House Ave., Frederick, MD 21701. Phone: (301) 846-7677. Fax: (301) 846-6100. E-mail: forestwu{at}mail.nih.gov.

Journal of Virology, April 2005, p. 5211-5214, Vol. 79, No. 8
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.8.5211-5214.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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