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Journal of Virology, April 2005, p. 4407-4414, Vol. 79, No. 7
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.7.4407-4414.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Internal Point Mutations of the Capsid Modify the Serotype of Rice Yellow Mottle Virus

Eugénie Hébrard,1* Agnès Pinel-Galzi,1 Vincent Catherinot,2 Gilles Labesse,2 Christophe Brugidou,1 and Denis Fargette1

Institut de Recherche pour le Développement (IRD),1 Centre de Biochimie Structurale, CNRS UMR5048-INSERM U554, Faculté de Pharmacie, Montpellier, France2

Received 1 July 2004/ Accepted 21 October 2004

Rice yellow mottle virus is classified in five major serotypes; the molecular diversity of the coat protein (CP) is well established, but the amino acids involved in the recognition by discriminant monoclonal antibodies (MAbs) remain unknown. Reconstruction of a phylogenetic tree and sequence alignment of the CP gene of a sample representative of the continental-large diversity were used to identify 10 serospecific amino acids (i.e., conserved in all isolates belonging to the same serotype and distinct in other serotypes). Positions occupied by serospecific residues were localized on the crystal structure of the CP monomer and on modeled capsomers. Structural, molecular, and serological properties of each serotype were analyzed, and subsequently, hypotheses on the potential role of amino acids in discriminating reactions with antibodies were formulated. The residues 114 and 115 (serospecific of Sr1) and 190 (serospecific of Sr2) were localized on the outer surface of the capsid and might be directly involved in the immunoreactivity with MAb D and MAb A, respectively. In contrast, residues 180 (Sr3) and 178 (Sr5) lay within the inner surface of the capsid. To understand the role of these internal positions in the recognition with the antibodies, two substitutions (T180K and G178D) were introduced in the CP of an infectious clone. These mutations modified the antigenicity with MAb G and MAb E discriminating Sr3 and Sr5, respectively, while the reaction with MAb D remained unaffected. This result suggests an indirect effect of these two internal mutations on local immunostructure while the global structure was maintained.

* Corresponding author. Mailing address: IRD BP 64501, 34394 Montpellier cedex 5, France. Phone: 33 4 67 41 62 89. Fax: 33 4 67 41 62 83. E-mail: hebrard{at}mpl.ird.fr.

Journal of Virology, April 2005, p. 4407-4414, Vol. 79, No. 7
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.7.4407-4414.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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