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Journal of Virology, March 2005, p. 3459-3467, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3459-3467.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Glycoprotein M Is an Essential Lytic Replication Protein of the Murine Gammaherpesvirus 68

Janet S. May, Susanna Colaco, and Philip G. Stevenson*

Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom

Received 28 July 2004/ Accepted 28 October 2004

All herpesviruses encode a homolog of glycoprotein M (gM), which appears to function in virion morphogenesis. Despite its conservation, gM is inessential for the lytic replication of alphaherpesviruses. In order to address the importance of gM in gammaherpesviruses, we disrupted it in the murine gammaherpesvirus 68 (MHV-68). The mutant virus completely failed to propagate in normally permissive fibroblasts. The defective genome was rescued by either homologous recombination to restore the wild-type gM in situ or the insertion of an ectopic, intergenic expression cassette encoding gM into the viral genome. Thus, gM was essential for the lytic replication of MHV-68.

* Corresponding author. Mailing address: Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United Kingdom. Phone: 44-1223-336921. Fax: 44-1223-336926. E-mail:pgs27{at}mole.bio.cam.ac.uk.

Journal of Virology, March 2005, p. 3459-3467, Vol. 79, No. 6
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.6.3459-3467.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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