This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schmitt, A. P.
Right arrow Articles by Lamb, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmitt, A. P.
Right arrow Articles by Lamb, R. A.

 Previous Article  |  Next Article 

Journal of Virology, March 2005, p. 2988-2997, Vol. 79, No. 5
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.5.2988-2997.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evidence for a New Viral Late-Domain Core Sequence, FPIV, Necessary for Budding of a Paramyxovirus

Anthony P. Schmitt,1 George P. Leser,1 Eiji Morita,2 Wesley I. Sundquist,2 and Robert A. Lamb1,3*

Department of Biochemistry, Molecular Biology and Cell Biology,1 Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois,3 Department of Biochemistry, University of Utah, Salt Lake City, Utah2

Received 7 September 2004/ Accepted 13 October 2004

Enveloped virus budding has been linked to both the ubiquitin-proteasome pathway and the vacuolar protein-sorting pathway of cells. We show here for the paramyxovirus SV5 that proteasome inhibitors and expression of dominant-negative VPS4(E228Q) ATPase blocks budding. The SV5 matrix (M) protein lacks previously defined late domains (e.g., P[T/S]AP, PPxY, YPDL) that recruit cellular factors. We identified a new motif for budding (core sequence FPIV) that can compensate functionally for lack of a PTAP late domain in budding human immunodeficiency virus type 1 virus-like particles (VLPs). Mutagenesis experiments suggest the more general sequence Ø-P-x-V. The proline residue was found to be critically important for function of this sequence, as substitution of this proline in the SV5 M protein resulted in poor budding of SV5 VLPs and failure of recombinant SV5 virus to replicate normally. Adaptation of mutant virus occurred rapidly, resulting in new proline residues elsewhere in the M protein. We hypothesize that these proline residues act to partially restore virus budding by generation of new motifs that act as suboptimal late domains.

* Corresponding author. Mailing address: BMBCB, Northwestern University, 2205 Tech Dr., Evanston, IL 60208-3500. Phone: (847) 491-5433. Fax: (847) 491-2467. E-mail: ralamb{at}northwestern.edu.

Journal of Virology, March 2005, p. 2988-2997, Vol. 79, No. 5
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.5.2988-2997.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, M., Schmitt, P. T., Li, Z., McCrory, T. S., He, B., Schmitt, A. P. (2009). Mumps Virus Matrix, Fusion, and Nucleocapsid Proteins Cooperate for Efficient Production of Virus-Like Particles. J. Virol. 83: 7261-7272 [Abstract] [Full Text]  
  • McDonald, B., Martin-Serrano, J. (2009). No strings attached: the ESCRT machinery in viral budding and cytokinesis. J. Cell Sci. 122: 2167-2177 [Abstract] [Full Text]  
  • Kolesnikova, L., Strecker, T., Morita, E., Zielecki, F., Mittler, E., Crump, C., Becker, S. (2009). Vacuolar Protein Sorting Pathway Contributes to the Release of Marburg Virus. J. Virol. 83: 2327-2337 [Abstract] [Full Text]  
  • Agromayor, M., Carlton, J. G., Phelan, J. P., Matthews, D. R., Carlin, L. M., Ameer-Beg, S., Bowers, K., Martin-Serrano, J. (2009). Essential Role of hIST1 in Cytokinesis. Mol. Biol. Cell 20: 1374-1387 [Abstract] [Full Text]  
  • Utley, T. J., Ducharme, N. A., Varthakavi, V., Shepherd, B. E., Santangelo, P. J., Lindquist, M. E., Goldenring, J. R., Crowe, J. E. Jr. (2008). Respiratory syncytial virus uses a Vps4-independent budding mechanism controlled by Rab11-FIP2. Proc. Natl. Acad. Sci. USA 105: 10209-10214 [Abstract] [Full Text]  
  • Ushijima, Y., Koshizuka, T., Goshima, F., Kimura, H., Nishiyama, Y. (2008). Herpes Simplex Virus Type 2 UL56 Interacts with the Ubiquitin Ligase Nedd4 and Increases Its Ubiquitination. J. Virol. 82: 5220-5233 [Abstract] [Full Text]  
  • Taylor, G. M., Hanson, P. I., Kielian, M. (2007). Ubiquitin Depletion and Dominant-Negative VPS4 Inhibit Rhabdovirus Budding without Affecting Alphavirus Budding. J. Virol. 81: 13631-13639 [Abstract] [Full Text]  
  • Zhadina, M., McClure, M. O., Johnson, M. C., Bieniasz, P. D. (2007). Ubiquitin-dependent virus particle budding without viral protein ubiquitination. Proc. Natl. Acad. Sci. USA 104: 20031-20036 [Abstract] [Full Text]  
  • Jager, S., Gottwein, E., Krausslich, H.-G. (2007). Ubiquitination of Human Immunodeficiency Virus Type 1 Gag Is Highly Dependent on Gag Membrane Association. J. Virol. 81: 9193-9201 [Abstract] [Full Text]  
  • Chen, B. J., Leser, G. P., Morita, E., Lamb, R. A. (2007). Influenza Virus Hemagglutinin and Neuraminidase, but Not the Matrix Protein, Are Required for Assembly and Budding of Plasmid-Derived Virus-Like Particles. J. Virol. 81: 7111-7123 [Abstract] [Full Text]  
  • Watanabe, T., Sorensen, E. M., Naito, A., Schott, M., Kim, S., Ahlquist, P. (2007). Involvement of host cellular multivesicular body functions in hepatitis B virus budding. Proc. Natl. Acad. Sci. USA 104: 10205-10210 [Abstract] [Full Text]  
  • Saunders, A. A., Ting, J. P. C., Meisner, J., Neuman, B. W., Perez, M., de la Torre, J. C., Buchmeier, M. J. (2007). Mapping the Landscape of the Lymphocytic Choriomeningitis Virus Stable Signal Peptide Reveals Novel Functional Domains. J. Virol. 81: 5649-5657 [Abstract] [Full Text]  
  • Pohl, C., Duprex, W. P., Krohne, G., Rima, B. K., Schneider-Schaulies, S. (2007). Measles virus M and F proteins associate with detergent-resistant membrane fractions and promote formation of virus-like particles. J. Gen. Virol. 88: 1243-1250 [Abstract] [Full Text]  
  • Irie, T., Shimazu, Y., Yoshida, T., Sakaguchi, T. (2007). The YLDL Sequence within Sendai Virus M Protein Is Critical for Budding of Virus-Like Particles and Interacts with Alix/AIP1 Independently of C Protein. J. Virol. 81: 2263-2273 [Abstract] [Full Text]  
  • Ciancanelli, M. J., Basler, C. F. (2006). Mutation of YMYL in the Nipah Virus Matrix Protein Abrogates Budding and Alters Subcellular Localization. J. Virol. 80: 12070-12078 [Abstract] [Full Text]  
  • Zamborlini, A., Usami, Y., Radoshitzky, S. R., Popova, E., Palu, G., Gottlinger, H. (2006). Release of autoinhibition converts ESCRT-III components into potent inhibitors of HIV-1 budding. Proc. Natl. Acad. Sci. USA 103: 19140-19145 [Abstract] [Full Text]  
  • Pantua, H. D., McGinnes, L. W., Peeples, M. E., Morrison, T. G. (2006). Requirements for the Assembly and Release of Newcastle Disease Virus-Like Particles. J. Virol. 80: 11062-11073 [Abstract] [Full Text]  
  • McCown, M. F., Pekosz, A. (2006). Distinct domains of the influenza a virus m2 protein cytoplasmic tail mediate binding to the m1 protein and facilitate infectious virus production.. J. Virol. 80: 8178-8189 [Abstract] [Full Text]  
  • Urata, S., Noda, T., Kawaoka, Y., Yokosawa, H., Yasuda, J. (2006). Cellular factors required for lassa virus budding.. J. Virol. 80: 4191-4195 [Abstract] [Full Text]  
  • Hui, E. K.-W., Barman, S., Tang, D. H.-P., France, B., Nayak, D. P. (2006). YRKL Sequence of Influenza Virus M1 Functions as the L Domain Motif and Interacts with VPS28 and Cdc42. J. Virol. 80: 2291-2308 [Abstract] [Full Text]  
  • Wirblich, C., Bhattacharya, B., Roy, P. (2006). Nonstructural Protein 3 of Bluetongue Virus Assists Virus Release by Recruiting ESCRT-I Protein Tsg101. J. Virol. 80: 460-473 [Abstract] [Full Text]  
  • Irie, T., Harty, R. N. (2005). L-Domain Flanking Sequences Are Important for Host Interactions and Efficient Budding of Vesicular Stomatitis Virus Recombinants. J. Virol. 79: 12617-12622 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»