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Journal of Virology, February 2005, p. 2614-2619, Vol. 79, No. 4
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.4.2614-2619.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Inhibition of Virus DNA Replication by Artificial Zinc Finger Proteins

Takashi Sera1*

Torrey Mesa Research Institute, San Diego, California1

Received 25 July 2004/ Accepted 5 October 2004

Prevention of virus infections is a major objective in agriculture and human health. One attractive approach to the prevention is inhibition of virus replication. To demonstrate this concept in vivo, an artificial zinc finger protein (AZP) targeting the replication origin of the Beet severe curly top virus (BSCTV), a model DNA virus, was created. In vitro DNA binding assays indicated that the AZP efficiently blocked binding of the viral replication protein (Rep), which initiates virus replication, to the replication origin. All of the transgenic Arabidopsis plants expressing the AZP showed phenotypes strongly resistant to virus infection, and 84% of the transgenic plants showed no symptom. Southern blot analysis demonstrated that BSCTV replication was completely suppressed in the transgenic plants. Since the mechanism of viral DNA replication is well conserved among plants and mammals, this approach could be applied not only to agricultural crop protection but also to the prevention of virus infections in humans.

* Present address: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyotodaigaku-Katsura, Nishikyo-Ku, Kyoto 615-8510, Japan. Phone: 81-75-383-2769. Fax: 81-75-383-2767. E-mail: sera{at}sbchem.kyoto-u.ac.jp.

Journal of Virology, February 2005, p. 2614-2619, Vol. 79, No. 4
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.4.2614-2619.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mino, T., Hatono, T., Matsumoto, N., Mori, T., Mineta, Y., Aoyama, Y., Sera, T. (2006). Inhibition of DNA replication of human papillomavirus by artificial zinc finger proteins.. J. Virol. 80: 5405-5412 [Abstract] [Full Text]  
  • Lopez-Ochoa, L., Ramirez-Prado, J., Hanley-Bowdoin, L. (2006). Peptide Aptamers That Bind to a Geminivirus Replication Protein Interfere with Viral Replication in Plant Cells.. J. Virol. 80: 5841-5853 [Abstract] [Full Text]  
  • Delmer, D. P. (2005). Inaugural Article: Agriculture in the developing world: Connecting innovations in plant research to downstream applications. Proc. Natl. Acad. Sci. USA 102: 15739-15746 [Abstract] [Full Text]  


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