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Journal of Virology, February 2005, p. 1970-1974, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1970-1974.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Atomic Force Microscopy Investigation of Isolated Virions of Murine Leukemia Virus

Y. G. Kuznetsov, A. Low, H. Fan, and A. McPherson*

Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, California

Received 14 July 2004/ Accepted 15 September 2004

Virions of mouse leukemia virus spread on glass substrates were visualized by atomic force microscopy. The size distribution mode was 145 nm, significantly larger than that for human immunodeficiency virus particles. The distribution of particle sizes is broad, indicating that no two particles are likely identical in content or surface features. Virions possess knoblike protrusions, which may represent vestiges of budding from cell membranes. Particles which split open allowed imaging of intact cores with diameters of 65 nm. They also permitted estimation of viral shell thickness (35 to 40 nm) and showed the presence of a distinct trough between the shell and the core surface.

* Corresponding author. Mailing address: Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697-3900. Phone: (949) 824-1931. Fax: (949) 824-5992. E-mail: amcphers{at}uci.edu.

Journal of Virology, February 2005, p. 1970-1974, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1970-1974.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Low, A., Datta, S., Kuznetsov, Y., Jahid, S., Kothari, N., McPherson, A., Fan, H. (2007). Mutation in the Glycosylated Gag Protein of Murine Leukemia Virus Results in Reduced In Vivo Infectivity and a Novel Defect in Viral Budding or Release. J. Virol. 81: 3685-3692 [Abstract] [Full Text]  


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