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Journal of Virology, February 2005, p. 1948-1953, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1948-1953.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Bovine Herpesvirus 1 VP22 Enhances the Efficacy of a DNA Vaccine in Cattle{dagger}

Chunfu Zheng, Lorne A. Babiuk, and Sylvia van Drunen Littel-van den Hurk*

Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

Received 7 April 2004/ Accepted 7 September 2004

For this study, the intercellular trafficking ability of bovine herpesvirus 1 (BHV-1) VP22 was applied to improve the efficacy of a DNA vaccine in calves. A plasmid encoding a truncated version of glycoprotein D (tgD) fused to VP22 was constructed. The plasmid encoding tgD-VP22 elicited significantly enhanced and more balanced immune responses than those induced by a plasmid encoding tgD. Furthermore, protection against a BHV-1 challenge was obtained in calves immunized with the plasmid encoding tgD-VP22, as shown by significant reductions in viral excretion. However, less significant protection was observed for animals vaccinated with the tgD-expressing plasmid, correlating with the lower level of immunity observed prechallenge. This is the first report of the use of VP22 as a transport molecule in the context of a DNA vaccine for a large animal species.

* Corresponding author. Mailing address: Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd., Saskatoon, SK S7N 5E3, Canada. Phone: (306) 966-1559. Fax: (306) 966-7478. E-mail: vandenhurk{at}sask.usask.ca.

{dagger} VIDO's Journal Series number 370.

Journal of Virology, February 2005, p. 1948-1953, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1948-1953.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yao, W., Liu, S., Qu, X., Xiao, S., Liu, Y., Liu, J. (2009). Enhanced immune response and protection efficacy of a DNA vaccine constructed by linkage of the Mycobacterium tuberculosis Ag85B-encoding gene with the BVP22-encoding gene. J Med Microbiol 58: 462-468 [Abstract] [Full Text]  
  • Zheng, C., Brownlie, R., Babiuk, L. A., van Drunen Littel-van den Hurk, S. (2005). Characterization of the Nuclear Localization and Nuclear Export Signals of Bovine Herpesvirus 1 VP22. J. Virol. 79: 11864-11872 [Abstract] [Full Text]  


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