This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Horn, G. P.
Right arrow Articles by Dobbelstein, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Horn, G. P.
Right arrow Articles by Dobbelstein, M.

 Previous Article  |  Next Article 

Journal of Virology, February 2005, p. 1911-1917, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1911-1917.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Enhanced Cytotoxicity without Internuclear Spread of Adenovirus upon Cell Fusion by Measles Virus Glycoproteins

German P. Horn,1,2 Sompong Vongpunsawad,3 Evelyn Kornmann,4 Barbara Fritz,4 Dirk P. Dittmer,5 Roberto Cattaneo,3 and Matthias Dobbelstein1,6*

Institut für Virologie,1 Abteilung Gastroenterologie, Stoffwechsel und Endokrinologie,2 Zentrum für Humangenetik, Klinikum der Philipps, Universität Marburg, Marburg, Germany,4 Molecular Medicine Program, Mayo Clinic, and Virology and Gene Therapy, Mayo Graduate School, Rochester, Minnesota,3 Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina,5 Medical Biotechnology Center, University of Southern Denmark, Odense, Denmark6

Received 5 July 2004/ Accepted 10 September 2004

The efficiency of viruses in cancer therapy is enhanced by proteins that mediate the fusion of infected cells with their neighbors. It was reported that replication-competent adenovirus particles can spread between nuclei within fusion-generated syncytia. To assess this conjecture, we generated fusogenic adenoviruses that express a balanced ratio of the F and H glycoproteins of measles virus. The viruses displayed enhanced cytotoxicity but largely unchanged replication efficiencies compared to a nonfusogenic virus. Most notably, the virus genomes did not spread through fusion-generated multinuclear cells. Hence, adenovirus replication in syncytia remains largely restricted to initially transduced nuclei.

* Corresponding author. Present address: Medical Biotechnology Center, University of Southern Denmark, Winsløwparken 25, 5000 Odense C, Denmark. Phone: 45 6550 3970. Fax: 45 6550 3950. E-mail: MDobbelstein{at}health.sdu.dk.

Journal of Virology, February 2005, p. 1911-1917, Vol. 79, No. 3
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.3.1911-1917.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hoffmann, D., Wildner, O. (2006). Enhanced killing of pancreatic cancer cells by expression of fusogenic membrane glycoproteins in combination with chemotherapy.. Molecular Cancer Therapeutics 5: 2013-2022 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»