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Journal of Virology, December 2005, p. 15582-15585, Vol. 79, No. 24
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.24.15582-15585.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Deubiquitinating Activity Is Conserved in the Large Tegument Protein of the Herpesviridae

Christian Schlieker,{dagger} Gregory A. Korbel,{dagger} Lisa M. Kattenhorn,{dagger} and Hidde L. Ploegh*

Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, NRB, Boston, Massachusetts 02115

Received 16 August 2005/ Accepted 20 September 2005

The largest tegument protein of herpes simplex virus 1 (HSV-1), UL36, contains a novel deubiquitinating activity embedded in it. All members of the Herpesviridae contain a homologue of HSV-1 UL36, the N-terminal segments of which show perfect conservation of those residues implicated in catalysis. For murine cytomegalovirus and Epstein-Barr virus, chosen as representatives of the beta- and gammaherpesvirus subfamilies, respectively, we here show that the homologous modules indeed display deubiquitinating activity in vitro. The conservation of this activity throughout all subfamilies is indicative of an important, if not essential, function.

* Corresponding author. Present address: Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142. Phone: (617) 324-1878. Fax: (617) 452-3566. E-mail: ploegh{at}wi.mit.edu.

{dagger} Present address: Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142.

Journal of Virology, December 2005, p. 15582-15585, Vol. 79, No. 24
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.24.15582-15585.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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