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Journal of Virology, December 2005, p. 14962-14966, Vol. 79, No. 23
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.23.14962-14966.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Two Residues within the LDL-A Module of Tva That Dictate the Altered Receptor Specificity of Mutant Subgroup A Avian Sarcoma and Leukosis Viruses

Tia Rai,1 Michael Caffrey,2 and Lijun Rong1*

Department of Microbiology and Immunology,1 Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 606122

Received 2 June 2005/ Accepted 15 September 2005

Avian sarcoma and leukosis virus subgroup A (ASLV-A) entry is mediated by interactions between the viral glycoprotein EnvA and its cognate receptor Tva. Previously, some interesting mutants of ASLV-A have been selected by others which can use chicken Tva, but not quail Tva, for efficient entry. The mutant phenotypes are caused by two point mutations within the surface subunit of EnvA (S. L. Holmen, D. C. Melder, and M. J. Federspiel, J. Virol. 75:726-737, 2001). In this study, we have shown that the altered receptor specificity maps to the LDL-A module of Tva. Further, we have identified two residues in the chicken LDL-A module that allow more efficient viral entry by the mutant viruses. These results demonstrate that the altered receptor specificity of the mutant viruses is determined by specific interactions with residues in the LDL-A module of Tva.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, 8133 COMRB, 909 S. Wolcott Ave., Chicago, IL 60612. Phone: (312) 355-0203. Fax: (312) 355-0220. E-mail: lijun{at}uic.edu.

Journal of Virology, December 2005, p. 14962-14966, Vol. 79, No. 23
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.23.14962-14966.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Munguia, A., Federspiel, M. J. (2008). Efficient Subgroup C Avian Sarcoma and Leukosis Virus Receptor Activity Requires the IgV Domain of the Tvc Receptor and Proper Display on the Cell Membrane. J. Virol. 82: 11419-11428 [Abstract] [Full Text]  
  • Scott, D. J., Layfield, S., Yan, Y., Sudo, S., Hsueh, A. J. W., Tregear, G. W., Bathgate, R. A. D. (2006). Characterization of Novel Splice Variants of LGR7 and LGR8 Reveals That Receptor Signaling Is Mediated by Their Unique Low Density Lipoprotein Class A Modules. J. Biol. Chem. 281: 34942-34954 [Abstract] [Full Text]  
  • Contreras-Alcantara, S., Godby, J. A., Delos, S. E. (2006). The Single Ligand-binding Repeat of Tva, a Low Density Lipoprotein Receptor-related Protein, Contains Two Ligand-binding Surfaces. J. Biol. Chem. 281: 22827-22838 [Abstract] [Full Text]  


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