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Journal of Virology, November 2005, p. 14442-14445, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14442-14445.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

An N-Linked Glycoprotein with {alpha}(2,3)-Linked Sialic Acid Is a Receptor for BK Virus

Aisling S. Dugan,1,2 Sylvia Eash,1,2 and Walter J. Atwood2*

Graduate Program in Pathobiology,1 Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island2

Received 23 June 2005/ Accepted 12 August 2005

BK virus (BKV) is a common human polyomavirus infecting >80% of the population worldwide. Infection with BKV is asymptomatic, but reactivation in renal transplant recipients can lead to polyomavirus-associated nephropathy. In this report, we show that enzymatic removal of {alpha}(2,3)-linked sialic acid from cells inhibited BKV infection. Reconstitution of asialo cells with {alpha}(2,3)-specific sialyltransferase restored susceptibility to infection. Inhibition of N-linked glycosylation with tunicamycin reduced infection, but inhibition of O-linked glycosylation did not. An O-linked-specific {alpha}(2,3)-sialyltransferase was unable to restore infection in asialo cells. Taken together, these data indicate that an N-linked glycoprotein containing {alpha}(2,3)-linked sialic acid is a critical component of the cellular receptor for BKV.

* Corresponding author. Mailing address: Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, 70 Ship St., Providence, RI 02903. Phone: (401) 863-3116. Fax: (401) 863-9653. E-mail: Walter_Atwood{at}Brown.edu.

Journal of Virology, November 2005, p. 14442-14445, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14442-14445.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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