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Journal of Virology, November 2005, p. 14437-14441, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14437-14441.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Intracellular Interference of Infectious Bursal Disease Virus

Dolores González, Jose Francisco Rodríguez,* and Fernando Abaitua{dagger}

Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología, CSIC, Calle Darwin n° 3, 28049 Madrid, Spain

Received 2 June 2005/ Accepted 24 August 2005

A search for dominant-negative mutant polypeptides hampering infectious bursal disease virus (IBDV) replication has been undertaken. We have found that expression of a mutant version of the VP3 structural polypeptide known as VP3/M3, partially lacking the domain responsible for the interaction with the virus-encoded RNA polymerase, efficiently interferes with the IBDV replication cycle. Transformed cells stably expressing VP3/M3 show a significant reduction (up to 96%) in their ability to support IBDV growth. Our findings provide a new tool for the characterization of the IBDV replication cycle and might facilitate the generation of genetically modified chicken lines with a reduced susceptibility to IBDV infection.

* Corresponding author. Mailing address: Departamento de Biología Molecular y Celular, Centro Nacional de Biotecnología, Calle Darwin n° 3, 28049 Madrid, Spain. Phone: 34-915854558. Fax: 34-915854506. E-mail: jfrodrig{at}cnb.uam.es.

{dagger} Present address: Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom.

Journal of Virology, November 2005, p. 14437-14441, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14437-14441.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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