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Journal of Virology, November 2005, p. 14383-14391, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14383-14391.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Establishment and Maintenance of Kaposi's Sarcoma-Associated Herpesvirus Latency in B Cells

Lei Chen and Michael Lagunoff*

Department of Microbiology, University of Washington, Seattle, Washington 98195

Received 1 June 2005/ Accepted 24 August 2005

Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) is the infectious cause of Kaposi's sarcoma and is also associated with two B-cell lymphoproliferative diseases, primary effusion lymphoma and the plasmablastic form of multicentric Castleman's disease. KSHV is also found in the B-cell fraction of peripheral blood mononucleocytes of some KS patients. Despite in vivo infection of B cells and the ability of KSHV to infect many cell types in culture, to date B cells in culture have been resistant to KSHV infection. However, as shown here, the lack of infection is not due to the inability of B cells to support latent KSHV infection. When KSHV DNA is introduced into B cells, the virus is maintained as an episome and can establish and maintain latency over the course of months. As in all primary effusion lymphoma cell lines, there is a low level of spontaneous lytic replication in latently infected BJAB cells. Importantly, viral gene expression is similar to that of primary effusion lymphoma cell lines. Furthermore, the virus can be reactivated to higher levels with specific stimuli and transmitted to other cells, indicating that this is a productive infection. Thus B cells in culture are capable of establishing, maintaining, and reactivating from latency. These studies provide a controlled system to analyze how KSHV alters B cells during KSHV latency and reactivation.

* Corresponding author. Mailing address: University of Washington, Department of Microbiology, 1959 N.E. Pacific St., HSB H-310J, Box 357242, Seattle WA 98195. Phone: (206) 616-4285. Fax: (206) 543-8297. E-mail: Lagunoff{at}u.washington.edu.

Journal of Virology, November 2005, p. 14383-14391, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14383-14391.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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