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Journal of Virology, November 2005, p. 14197-14206, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14197-14206.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characterization of Parvovirus B19 Genotype 2 in KU812Ep6 Cells

Johannes Blümel,1* Anna Maria Eis-Hübinger,2 Albert Stühler,1 Claudia Bönsch,1 Matthias Gessner,3 and Johannes Löwer1

Paul-Ehrlich-Institut, 63225 Langen,1 Institut für Medizinische Mikrobiologie und Immunologie, Universität Bonn, 53127 Bonn, Germany,2 Baxter Bioscience, Vienna, Austria3

Received 30 May 2005/ Accepted 11 August 2005

An infectious parvovirus B19 (B19V) genotype 2 variant was identified as a high-titer contaminant in a human plasma donation. Genome analysis revealed a 138-bp insertion within the p6 promoter. The inserted sequence was represented by an additional 30 bp from the end of the inverted terminal repeat adjacent to a 108-bp element found also, in inverted orientation, at the extreme right end of the unique sequence of the genome. However, despite the profound variations in the promoter region, the pattern of gene expression and DNA replication did not differ between genotype 1 and genotype 2 in permissive erythroid KU812Ep6 cells. Capsid proteins of both genotypes differ in their amino acid sequences. However, equivalent kinetics of virus inactivation at 56°C or pH 4 indicated a comparable physicochemical stability of virus capsids. Sera from six individuals infected by B19V genotype 1 were investigated on cross-neutralization of B19V genotype 2 in vitro. Similar neutralization of both B19V genotypes was observed in sera from three individuals, while the sera from three other individuals showed weaker cross-neutralization for genotype 2. In conclusion, the in vitro replication characteristics and physical stability of B19V capsids are very similar between human parvovirus B19 genotypes 1 and 2, and cross-neutralization indicates a close antigenic relation of genotypes 1 and 2.

* Corresponding author. Mailing address: Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, 63225 Langen, Germany. Phone: 49-6103-773803. Fax: 49-6103-771265. E-mail: blujo{at}pei.de.

Journal of Virology, November 2005, p. 14197-14206, Vol. 79, No. 22
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.22.14197-14206.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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