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Journal of Virology, January 2005, p. 717-724, Vol. 79, No. 2
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.2.717-724.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Virus-Like Particles as Carriers for T-Cell Epitopes: Limited Inhibition of T-Cell Priming by Carrier-Specific Antibodies

Christiane Ruedl, Katrin Schwarz, Andrea Jegerlehner, Tazio Storni, Vania Manolova, and Martin F. Bachmann*

Cytos Biotechnology AG, Schlieren-Zürich, Switzerland

Received 15 July 2004/ Accepted 4 August 2004

Virus-like particles (VLPs) are able to induce cytotoxic T-cell responses in the absence of infection or replication. This makes VLPs promising candidates for the development of recombinant vaccines. However, VLPs are also potent inducers of B-cell responses, and it is generally assumed that such VLP-specific antibodies interfere with the induction of protective immune responses, a phenomenon summarized as carrier suppression. In this study, we investigated the impact of preexisting VLP-specific antibodies on the induction of specific cytotoxic T-cell and Th-cell responses in mice. The data show that VLP-specific antibodies did not measurably reduce antigen presentation in vitro or in vivo. Nevertheless, T-cell priming was slightly reduced by antigen-specific antibodies; however, the overall reduction was limited and vaccination with VLPs in the presence of VLP-specific antibodies still resulted in protective T-cell responses. Thus, carrier suppression is unlikely to be a limiting factor for VLP-based T-cell vaccines.

* Corresponding author. Mailing address: Cytos Biotechnology AG, Wagistrasse 25, 8952 Schlieren-Zürich, Switzerland. Phone: 41 1 733 40 23. Fax: 41 1 733 47 04. E-mail: martin.bachmann{at}cytos.com.

Journal of Virology, January 2005, p. 717-724, Vol. 79, No. 2
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.2.717-724.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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