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Journal of Virology, January 2005, p. 1308-1311, Vol. 79, No. 2
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.2.1308-1311.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Productive Replication of Human Adenovirus Type 5 in Canine Cells

Vladimir V. Ternovoi,1 Long P. Le,1 Natalya Belousova,1 Bruce F. Smith,2 Gene P. Siegal,1,3 and David T. Curiel1*

Division of Human Gene Therapy, Departments of Medicine, Pathology, and Surgery, and Gene Therapy Center,1 Departments of Pathology, Cell Biology, and Surgery, University of Alabama at Birmingham, Birmingham,3 Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, Alabama2

Received 16 June 2004/ Accepted 3 September 2004

Development of immunocompetent patient-like models that allow direct analysis of human adenovirus-based conditionally replicative adenoviruses (CRAds) would be beneficial for the advancement of these oncolytic agents. To this end, we explored the possibility of cross-species replication of human adenovirus type 5 (Ad5) in canine cells. With a panel of canine tumor cell lines of both epithelial and mesenchymal derivations, we demonstrate that human Ad5 can productively infect canine cells. Since the biological behavior and clinical presentation of certain dog tumors closely resemble those of their human counterparts, our results raise the possibility of exploiting canine models for preclinical analysis of candidate CRAd agents designed for human virotherapy.

* Corresponding author. Mailing address: 901 19th St. South, BMR2-502, Birmingham, AL 35294. Phone: (205) 934-8627. Fax: (205) 975-7476. E-mail: curiel{at}uab.edu.

Journal of Virology, January 2005, p. 1308-1311, Vol. 79, No. 2
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.2.1308-1311.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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