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Journal of Virology, September 2005, p. 11501-11506, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11501-11506.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Replication of Varicella-Zoster Virus in Human Skin Organ Culture

Shannon L. Taylor and Jennifer F. Moffat*

Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, New York 13210

Received 18 April 2005/ Accepted 25 May 2005

Varicella-zoster virus (VZV) infection is restricted to humans, which hinders studies of its pathogenesis in rodent models of disease. To facilitate the study of VZV skin tropism, we developed an ex vivo system using human fetal skin organ culture (SOC). VZV replication was analyzed by plaque assay, transmission electron microscopy, and histology. The yield of infectious VZV from SOC increased ~100-fold over 6 days, virions were abundant, and lesions developed that contained VZV antigens and resembled varicella and zoster lesions. The SOC system for VZV replication has applications for testing virus mutants and antiviral drugs.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210. Phone: (315) 464-5454. Fax: (315) 464-4417. E-mail: moffatj{at}upstate.edu.

Journal of Virology, September 2005, p. 11501-11506, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11501-11506.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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