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Journal of Virology, September 2005, p. 11496-11500, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11496-11500.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Enhancement of Enveloped Virus Entry by Phosphatidylserine

David A. Coil1,2 and A. Dusty Miller1*

Division of Human Biology,1 Molecular and Cellular Biology Program, Fred Hutchinson Cancer Research Center, Seattle, Washington 981092

Received 5 May 2005/ Accepted 26 May 2005

Enveloped virus vectors are used in a wide variety of applications. We have discovered that treatment of cultured cells with phosphatidylserine (PS) liposomes can increase virus vector infection by up to 20-fold. This effect does not abrogate virus receptor requirements, is specific to PS compared to other phospholipids, and is limited to enveloped viruses. Furthermore, the enhancement of infection does not occur through increases in virus receptor levels or virus binding, indicating that virus fusion is enhanced. The liposomes are easily generated, store well, and allow enhanced infection with a variety of virus vectors and cell types.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Room C2-105, P.O. Box 19024, Seattle, WA 98109-1024. Phone: (206) 667-2890. Fax: (206) 667-6523. E-mail: dmiller{at}fhcrc.org.

Journal of Virology, September 2005, p. 11496-11500, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11496-11500.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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