This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qiu, J.
Right arrow Articles by Pintel, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qiu, J.
Right arrow Articles by Pintel, D.

 Previous Article  |  Next Article 

Journal of Virology, September 2005, p. 11035-11044, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11035-11044.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Expression Strategy of Goose Parvovirus Exhibits Features of both the Dependovirus and Parvovirus Genera

Jianming Qiu,1* Fang Cheng,1 Yuko Yoto,1 Zoltán Zádori,2 and David Pintel1

Department of Molecular Microbiology and Immunology, Life Sciences Center, University of Missouri—Columbia, School of Medicine, Columbia, Missouri,1 INRS—Institut Armand-Frappier, Université du Québec, Laval, Québec, Canada2

Received 20 April 2005/ Accepted 3 June 2005

The RNA transcription profile of the goose parvovirus (GPV) was determined, and it is a surprising hybrid of features of the Parvovirus and Dependovirus genera of the Parvovirinae subfamily of the Parvoviridae. Similar to the Dependovirus adeno-associated virus type 5, RNAs transcribed from the GPV upstream P9 promoter, which encode the viral nonstructural proteins, were polyadenylated at a high efficiency at a polyadenylation site [(pA)p] located within an intron in the center of the genome. Efficient usage of (pA)p required a downstream element that overlaps with the polypyrimidine tract of the A2 3' splice site of the central intron. An upstream element required for efficient use of (pA)p was also identified. RNAs transcribed from the P42 promoter, presumed to encode the viral capsid proteins, primarily extended through (pA)p and were polyadenylated at a site, (pA)d, located at the right end of the genome and ultimately spliced at a high efficiency. No promoter analogous to the Dependovirus P19 promoter was detected; however, similar to minute virus of mice and other members of the Parvovirus genus, a significant portion of pre-mRNAs generated from the P9 promoter were additionally spliced within the putative GPV Rep1 coding region and likely encode an additional, smaller, nonstructural protein. Also similar to members of the Parvovirus genus, detectable activity of the GPV P42 promoter was highly dependent on transactivation by the GPV Rep1 protein in a manner dependent on binding to a cis-element located in the P42 promoter.

* Corresponding author. Mailing address: 471b Life Sciences Center, 1201 E. Rollins Rd., Columbia, MO 65211-7310. Phone: (573) 882-3171. Fax: (573) 882-4287. E-mail: qiuj{at}missouri.edu.

Journal of Virology, September 2005, p. 11035-11044, Vol. 79, No. 17
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.17.11035-11044.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, L., Qiu, J., Pintel, D. J. (2009). The Choice of Translation Initiation Site of the Rep Proteins from Goose Parvovirus P9-Generated mRNA Is Governed by Splicing and the Nature of the Excised Intron. J. Virol. 83: 10264-10268 [Abstract] [Full Text]  
  • Guan, W., Wong, S., Zhi, N., Qiu, J. (2009). The Genome of Human Parvovirus B19 Can Replicate in Nonpermissive Cells with the Help of Adenovirus Genes and Produces Infectious Virus. J. Virol. 83: 9541-9553 [Abstract] [Full Text]  
  • Qiu, J., Cheng, F., Pintel, D. J. (2006). Expression profiles of bovine adeno-associated virus and avian adeno-associated virus display significant similarity to that of adeno-associated virus type 5.. J. Virol. 80: 5482-5493 [Abstract] [Full Text]  
  • Yoto, Y., Qiu, J., Pintel, D. J. (2006). Identification and Characterization of Two Internal Cleavage and Polyadenylation Sites of Parvovirus B19 RNA. J. Virol. 80: 1604-1609 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»