This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Choi, Y. K.
Right arrow Articles by Webster, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Choi, Y. K.
Right arrow Articles by Webster, R. G.

 Previous Article  |  Next Article 

Journal of Virology, August 2005, p. 10821-10825, Vol. 79, No. 16
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.16.10821-10825.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Studies of H5N1 Influenza Virus Infection of Pigs by Using Viruses Isolated in Vietnam and Thailand in 2004

Young Ki Choi,1,7 Tien Dzung Nguyen,2 Hiroichi Ozaki,1,8 Richard J. Webby,1 Pilaipan Puthavathana,3 Chantanee Buranathal,4 Arunee Chaisingh,3 Prasert Auewarakul,4 N. T. H. Hanh,5 Sia Kit Ma,6 Pui Yan Hui,6 Yi Guan,6 Joseph Sriyal Malik Peiris,6 and Robert G. Webster1*

Division of Virology, Department of Infectious Diseases, St. Jude Children's Research Hospital, 332 N. Lauderdale St., Memphis, Tennessee 38105,1 Department of Virology, National Institute of Veterinary Research, Ministry of Agriculture and Rural Development, Hanoi, Vietnam,2 Department of Microbiology, Sriraj Hospital, Bangkok 10700, Thailand,3 Department of Livestock Development, National Institute of Animal Health, Bangkok 10900, Thailand,4 Virology Department, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam,5 Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China,6 College of Medicine and Medical Research Institute, Chungbuk National University, 12, Gaeshin-Dong Heungduk-Ku, Cheongju 361-763, Republic of Korea,7 Division of Project Research, Creative Research Initiative, Hokkaido University, Kita 21, Nishi 10, Kita-ku, Sapporo 001-0021, Japan8

Received 20 January 2005/ Accepted 1 April 2005

To determine whether avian H5N1 influenza viruses associated with human infections in Vietnam had transmitted to pigs, we investigated serologic evidence of exposure to H5N1 influenza virus in Vietnamese pigs in 2004. Of the 3,175 pig sera tested, 8 (0.25%) were positive for avian H5N1 influenza viruses isolated in 2004 by virus neutralization assay and Western blot analysis. Experimental studies of replication and transmissibility of the 2004 Asian H5N1 viruses in pigs revealed that all viruses tested replicated in the swine respiratory tract but none were transmitted to contact pigs. Virus titers from nasal swabs peaked on day 2, and low titers were detected in the liver of two of the four pigs tested. Our findings indicate that pigs can be infected with highly lethal Asian H5N1 viruses but that these viruses are not readily transmitted between pigs under experimental conditions.

* Corresponding author. Mailing address: Division of Virology, Department of Infectious Diseases, Mail Stop 330, St. Jude Children's Research Hospital, 332 N. Lauderdale St., Memphis, TN 38105-2794. Phone: (901) 495-3400. Fax: (901) 523-2622. E-mail: robert.webster{at}stjude.org.

Journal of Virology, August 2005, p. 10821-10825, Vol. 79, No. 16
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.16.10821-10825.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lee, J. H., Pascua, P. N. Q., Song, M.-S., Baek, Y. H., Kim, C.-J., Choi, H.-W., Sung, M.-H., Webby, R. J., Webster, R. G., Poo, H., Choi, Y. K. (2009). Isolation and Genetic Characterization of H5N2 Influenza Viruses from Pigs in Korea. J. Virol. 83: 4205-4215 [Abstract] [Full Text]  
  • Weingartl, H. M., Albrecht, R. A., Lager, K. M., Babiuk, S., Marszal, P., Neufeld, J., Embury-Hyatt, C., Lekcharoensuk, P., Tumpey, T. M., Garcia-Sastre, A., Richt, J. A. (2009). Experimental Infection of Pigs with the Human 1918 Pandemic Influenza Virus. J. Virol. 83: 4287-4296 [Abstract] [Full Text]  
  • Manzoor, R., Sakoda, Y., Nomura, N., Tsuda, Y., Ozaki, H., Okamatsu, M., Kida, H. (2009). PB2 Protein of a Highly Pathogenic Avian Influenza Virus Strain A/chicken/Yamaguchi/7/2004 (H5N1) Determines Its Replication Potential in Pigs. J. Virol. 83: 1572-1578 [Abstract] [Full Text]  
  • Gillim-Ross, L., Santos, C., Chen, Z., Aspelund, A., Yang, C.-F., Ye, D., Jin, H., Kemble, G., Subbarao, K. (2008). Avian Influenza H6 Viruses Productively Infect and Cause Illness in Mice and Ferrets. J. Virol. 82: 10854-10863 [Abstract] [Full Text]  
  • Vahlenkamp, T. W., Harder, T. C., Giese, M., Lin, F., Teifke, J. P., Klopfleisch, R., Hoffmann, R., Tarpey, I., Beer, M., Mettenleiter, T. C. (2008). Protection of cats against lethal influenza H5N1 challenge infection. J. Gen. Virol. 89: 968-974 [Abstract] [Full Text]  
  • van Riel, D., Munster, V. J., de Wit, E., Rimmelzwaan, G. F., Fouchier, R. A.M., Osterhaus, A. D.M.E., Kuiken, T. (2007). Human and Avian Influenza Viruses Target Different Cells in the Lower Respiratory Tract of Humans and Other Mammals. Am. J. Pathol. 171: 1215-1223 [Abstract] [Full Text]  
  • Mansfield, K. G. (2007). Viral Tropism and the Pathogenesis of Influenza in the Mammalian Host. Am. J. Pathol. 171: 1089-1092 [Full Text]  
  • Yen, H.-L., Lipatov, A. S., Ilyushina, N. A., Govorkova, E. A., Franks, J., Yilmaz, N., Douglas, A., Hay, A., Krauss, S., Rehg, J. E., Hoffmann, E., Webster, R. G. (2007). Inefficient Transmission of H5N1 Influenza Viruses in a Ferret Contact Model. J. Virol. 81: 6890-6898 [Abstract] [Full Text]  
  • Peiris, J. S. M., de Jong, M. D., Guan, Y. (2007). Avian Influenza Virus (H5N1): a Threat to Human Health. Clin. Microbiol. Rev. 20: 243-267 [Abstract] [Full Text]  
  • Roberton, S.I, Bell, D.J, Smith, G.J.D, Nicholls, J.M, Chan, K.H, Nguyen, D.T, Tran, P.Q, Streicher, U, Poon, L.L.M, Chen, H, Horby, P, Guardo, M, Guan, Y, Peiris, J.S.M (2006). Avian influenza H5N1 in viverrids: implications for wildlife health and conservation. Proc R Soc B 273: 1729-1732 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»