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Journal of Virology, August 2005, p. 9588-9596, Vol. 79, No. 15
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.15.9588-9596.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Utilization of Homotypic and Heterotypic Proteins of Vesicular Stomatitis Virus by Defective Interfering Particle Genomes for RNA Replication and Virion Assembly: Implications for the Mechanism of Homologous Viral Interference

Gyoung Nyoun Kim and C. Yong Kang*

Siebens-Drake Research Institute, The University of Western Ontario, London, Ontario, Canada N6G 2V4

Received 3 February 2005/ Accepted 20 April 2005

Defective interfering (DI) particles of Indiana serotype of vesicular stomatitis virus (VSVInd) are capable of interfering with the replication of both homotypic VSVInd and heterotypic New Jersey serotype (VSVNJ) standard virus. In contrast, DI particles from VSVNJ do not interfere with the replication of VSVInd standard virus but do interfere with VSVNJ replication. The differences in the interfering activities of VSVInd DI particles and VSVNJ DI particles against heterotypic standard virus were investigated. We examined the utilization of homotypic and heterotypic VSV proteins by DI particle genomic RNAs for replication and maturation into infectious DI particles. Here we show that the RNA-nucleocapsid protein (N) complex of one serotype does not utilize the polymerase complex (P and L) of the other serotype for RNA synthesis, while DI particle genomic RNAs of both serotypes can utilize the N, P, and L proteins of either serotype without serotypic restriction but with differing efficiencies as long as all three proteins are derived from the same serotype. The genomic RNAs of VSVInd DI particles assembled and matured into DI particles by using either homotypic or heterotypic viral proteins. In contrast, VSVNJ DI particles could assemble only with homotypic VSVNJ viral proteins, although the genomic RNAs of VSVNJ DI particles could be replicated by using heterotypic VSVInd N, P, and L proteins. Thus, we concluded that both efficient RNA replication and assembly of DI particles are required for the heterotypic interference by VSV DI particles.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Siebens-Drake Research Institute, The University of Western Ontario, 1400 Western Road, London, Ontario N6G 2V4, Canada. Phone: (519) 661-3226. Fax: (519) 661-3359. E-mail: cykang{at}uwo.ca.

Journal of Virology, August 2005, p. 9588-9596, Vol. 79, No. 15
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.15.9588-9596.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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