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Journal of Virology, July 2005, p. 9359-9362, Vol. 79, No. 14
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.14.9359-9362.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Reduced Mobilization of Rev-Responsive Element-Deficient Lentiviral Vectors

Susann Lucke, Thomas Grunwald, and Klaus Überla*

Department of Molecular and Medical Virology, Ruhr University, Bochum, Germany

Received 4 January 2005/ Accepted 16 March 2005

Infection of cells transduced with a lentiviral vector by human immunodeficiency virus (HIV) could lead to packaging of the lentiviral vector RNA into HIV particles and unintended transfer of the vector. To prevent this, the Rev-responsive element (RRE) of an HIV-1 vector was functionally replaced by a heterologous RNA element (MS2). Providing Rev fused to an MS2 binding protein allowed efficient vector production. Mobilization of the vector from infected target cells was below the level of detection and at least 103- to 104-fold lower than for the RRE-containing vector. Thus, RRE-deficient lentiviral vectors provide a novel approach to reduce the risk of vector mobilization.

* Corresponding author. Mailing address: Department of Molecular and Medical Virology, Ruhr University Bochum, D-44780 Bochum, Germany. Phone: 49-234-3223189. Fax: 49-234-3214352. E-mail: klaus.ueberla{at}ruhr-uni-bochum.de.

Journal of Virology, July 2005, p. 9359-9362, Vol. 79, No. 14
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.14.9359-9362.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Groom, H. C. T., Anderson, E. C., Lever, A. M. L. (2009). Rev: beyond nuclear export. J. Gen. Virol. 90: 1303-1318 [Abstract] [Full Text]  


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